2gtt

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Crystal structure of the rabies virus nucleoprotein-RNA complex

Structural highlights

2gtt is a 24 chain structure with sequence from Rabies virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Warning: this is a large structure, and loading might take a long time or not happen at all.

Function

[NCAP_RABVE] Encapsidates the genome in a ratio of one protein N per nine ribonucleotides, protecting it from nucleases. If expressed without protein P it binds non-specifically RNA and therefore can bind it's own mRNA. Interaction with protein P abolishes any non-specific RNA binding, and prevents phosphorylation. The soluble N-P complex encapsidates specifically the genomic RNA, with protein N protecting the genome like a pearl necklace. The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for viral transcription and replication. Protein N binds protein P in the NC through a different interaction, and can be phosphorylated. Subsequent viral replication is dependent on intracellular concentration of newly synthesized protein N. During replication, encapsidation by protein N is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity).

Publication Abstract from PubMed

Negative-strand RNA viruses condense their genome into a helical nucleoprotein-RNA complex, the nucleocapsid, which is packed into virions and serves as a template for the RNA-dependent RNA polymerase complex. The crystal structure of a recombinant rabies virus nucleoprotein-RNA complex, organized in an undecameric ring, has been determined at 3.5 angstrom resolution. Polymerization of the nucleoprotein is achieved by domain exchange between protomers, with flexible hinges allowing nucleocapsid formation. The two core domains of the nucleoprotein clamp around the RNA at their interface and shield it from the environment. RNA sequestering by nucleoproteins is likely a common mechanism used by negative-strand RNA viruses to protect their genomes from the innate immune response directed against viral RNA in human host cells at certain stages of an infectious cycle.

Crystal structure of the rabies virus nucleoprotein-RNA complex.,Albertini AA, Wernimont AK, Muziol T, Ravelli RB, Clapier CR, Schoehn G, Weissenhorn W, Ruigrok RW Science. 2006 Jul 21;313(5785):360-3. Epub 2006 Jun 15. PMID:16778023^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Albertini AA, Wernimont AK, Muziol T, Ravelli RB, Clapier CR, Schoehn G, Weissenhorn W, Ruigrok RW. Crystal structure of the rabies virus nucleoprotein-RNA complex. Science. 2006 Jul 21;313(5785):360-3. Epub 2006 Jun 15. PMID:16778023 doi:1125280

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gtt"

@@ Line 1: / Line 1: @@
+{{Large structure}}
 ==Crystal structure of the rabies virus nucleoprotein-RNA complex==
 <StructureSection load='2gtt' size='340' side='right' caption='[[2gtt]], [[Resolution|resolution]] 3.49&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2gtt]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Rabies_virus Rabies virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GTT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GTT FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gtt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gtt OCA], [http://pdbe.org/2gtt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gtt RCSB], [http://www.ebi.ac.uk/pdbsum/2gtt PDBsum]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gtt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gtt OCA], [http://pdbe.org/2gtt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gtt RCSB], [http://www.ebi.ac.uk/pdbsum/2gtt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2gtt ProSAT]</span></td></tr>
 </table>
 {{Large structure}}
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 </div>
 <div class="pdbe-citations 2gtt" style="background-color:#fffaf0;"></div>
-==See Also==
-*[[Nucleoprotein|Nucleoprotein]]
 == References ==
 <references/>

2gtt

From Proteopedia

Revision as of 08:01, 18 October 2017

Crystal structure of the rabies virus nucleoprotein-RNA complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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