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1zs4

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Revision as of 09:08, 2 May 2018

Structure of bacteriophage lambda cII protein in complex with DNA

Structural highlights

1zs4 is a 6 chain structure with sequence from Bacteriophage lambda. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1zpq
Gene:	CII (Bacteriophage lambda)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RPC2_LAMBD] This protein and protein CIII form a complex that is involved in the initiation of lysogeny. The complex binds at either of the cy regulatory sites preceding the CI repressor protein and integrase coding regions and induces transcription of these genes.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The tetrameric cII protein from bacteriophage lambda activates transcription from the phage promoters P(RE), P(I), and P(AQ) by binding to two direct repeats that flank the promoter -35 element. Here, we present the X-ray crystal structure of cII alone (2.8 A resolution) and in complex with its DNA operator from P(RE) (1.7 A resolution). The structures provide a basis for modeling of the activation complex with the RNA polymerase holoenzyme, and point to the key role for the RNA polymerase alpha subunit C-terminal domain (alphaCTD) in cII-dependent activation, which forms a bridge of protein/protein interactions between cII and the RNA polymerase sigma subunit. The model makes specific predictions for protein/protein interactions between cII and alphaCTD, and between alphaCTD and sigma, which are supported by previous genetic studies.

Crystal structure of bacteriophage lambda cII and its DNA complex.,Jain D, Kim Y, Maxwell KL, Beasley S, Zhang R, Gussin GN, Edwards AM, Darst SA Mol Cell. 2005 Jul 22;19(2):259-69. PMID:16039594^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Jain D, Kim Y, Maxwell KL, Beasley S, Zhang R, Gussin GN, Edwards AM, Darst SA. Crystal structure of bacteriophage lambda cII and its DNA complex. Mol Cell. 2005 Jul 22;19(2):259-69. PMID:16039594 doi:10.1016/j.molcel.2005.06.006

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1zs4"

@@ Line 1: / Line 1: @@
 ==Structure of bacteriophage lambda cII protein in complex with DNA==
 <StructureSection load='1zs4' size='340' side='right' caption='[[1zs4]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1zpq|1zpq]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Bacteriophage lambda])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zs4 OCA], [http://pdbe.org/1zs4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zs4 RCSB], [http://www.ebi.ac.uk/pdbsum/1zs4 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zs4 OCA], [http://pdbe.org/1zs4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zs4 RCSB], [http://www.ebi.ac.uk/pdbsum/1zs4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1zs4 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 13: / Line 14: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zs/1zs4_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zs/1zs4_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zs4 ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">

1zs4

From Proteopedia

Revision as of 09:08, 2 May 2018

Structure of bacteriophage lambda cII protein in complex with DNA

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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