This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

2lom

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 19:47, 1 August 2018

Backbone structure of human membrane protein HIGD1A

Structural highlights

2lom is a 1 chain structure with sequence from Human. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2lon, 2loo, 2lop, 2loq
Gene:	HIGD1A, HIG1, HSPC010 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[HIG1A_HUMAN] Proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes.^[1]

Publication Abstract from PubMed

Although nearly half of today's major pharmaceutical drugs target human integral membrane proteins (hIMPs), only 30 hIMP structures are currently available in the Protein Data Bank, largely owing to inefficiencies in protein production. Here we describe a strategy for the rapid structure determination of hIMPs, using solution NMR spectroscopy with systematically labeled proteins produced via cell-free expression. We report new backbone structures of six hIMPs, solved in only 18 months from 15 initial targets. Application of our protocols to an additional 135 hIMPs with molecular weight <30 kDa yielded 38 hIMPs suitable for structural characterization by solution NMR spectroscopy without additional optimization.

Facile backbone structure determination of human membrane proteins by NMR spectroscopy.,Klammt C, Maslennikov I, Bayrhuber M, Eichmann C, Vajpai N, Chiu EJ, Blain KY, Esquivies L, Kwon JH, Balana B, Pieper U, Sali A, Slesinger PA, Kwiatkowski W, Riek R, Choe S Nat Methods. 2012 May 20;9(8):834-9. doi: 10.1038/nmeth.2033. PMID:22609626^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Vukotic M, Oeljeklaus S, Wiese S, Vogtle FN, Meisinger C, Meyer HE, Zieseniss A, Katschinski DM, Jans DC, Jakobs S, Warscheid B, Rehling P, Deckers M. Rcf1 mediates cytochrome oxidase assembly and respirasome formation, revealing heterogeneity of the enzyme complex. Cell Metab. 2012 Mar 7;15(3):336-47. doi: 10.1016/j.cmet.2012.01.016. Epub 2012, Feb 16. PMID:22342701 doi:http://dx.doi.org/10.1016/j.cmet.2012.01.016
↑ Klammt C, Maslennikov I, Bayrhuber M, Eichmann C, Vajpai N, Chiu EJ, Blain KY, Esquivies L, Kwon JH, Balana B, Pieper U, Sali A, Slesinger PA, Kwiatkowski W, Riek R, Choe S. Facile backbone structure determination of human membrane proteins by NMR spectroscopy. Nat Methods. 2012 May 20;9(8):834-9. doi: 10.1038/nmeth.2033. PMID:22609626 doi:10.1038/nmeth.2033

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2lom"

@@ Line 1: / Line 1: @@
 ==Backbone structure of human membrane protein HIGD1A==
 <StructureSection load='2lom' size='340' side='right' caption='[[2lom]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2lon|2lon]], [[2loo|2loo]], [[2lop|2lop]], [[2loq|2loq]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HIGD1A, HIG1, HSPC010 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lom OCA], [http://pdbe.org/2lom PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lom RCSB], [http://www.ebi.ac.uk/pdbsum/2lom PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lom OCA], [http://pdbe.org/2lom PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lom RCSB], [http://www.ebi.ac.uk/pdbsum/2lom PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2lom ProSAT]</span></td></tr>
 </table>
 == Function ==

2lom

From Proteopedia

Revision as of 19:47, 1 August 2018

Backbone structure of human membrane protein HIGD1A

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox