1t2l

From Proteopedia

(Difference between revisions)

Revision as of 07:49, 14 March 2018

Three Crystal Structures of Human Coactosin-like Protein

Structural highlights

1t2l is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[COTL1_HUMAN] Binds to F-actin in a calcium-independent manner. Has no direct effect on actin depolymerization.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Human coactosin-like protein is an actin filament binding protein but does not bind to globular actin. It associates with 5-Lipoxygenase both in vivo and in vitro, playing important roles in modulating the activities of actin and 5-Lipoxygenase. Coactosin counteracts the capping activity of capping protein which inhibits the actin polymerization. We determined the crystal structures of human coactosin-like protein by multi-wavelength anomalous dispersion method. The structure showed a high level of similarity to ADF-H domain, although their amino acid sequences share low degree of homology. A few conserved hydrophobic residues that may contribute to the folding were identified. This structure suggests coactosin-like protein bind to F-actin in a different way from ADF/Cofilin family. Combined with the information from previous mutagenesis studies, the binding sites for F-actin and 5-Lipoxygenase were analyzed, respectively. These two sites are quite close, which might prevent F-actin and 5-Lipoxygenase from binding to coactosin simultaneously.

Crystal structure of human coactosin-like protein.,Liu L, Wei Z, Wang Y, Wan M, Cheng Z, Gong W J Mol Biol. 2004 Nov 19;344(2):317-23. PMID:15522287^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Provost P, Doucet J, Stock A, Gerisch G, Samuelsson B, Radmark O. Coactosin-like protein, a human F-actin-binding protein: critical role of lysine-75. Biochem J. 2001 Oct 15;359(Pt 2):255-63. PMID:11583571
↑ Liu L, Wei Z, Wang Y, Wan M, Cheng Z, Gong W. Crystal structure of human coactosin-like protein. J Mol Biol. 2004 Nov 19;344(2):317-23. PMID:15522287 doi:10.1016/j.jmb.2004.09.036

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1t2l"

@@ Line 1: / Line 1: @@
 ==Three Crystal Structures of Human Coactosin-like Protein==
 <StructureSection load='1t2l' size='340' side='right' caption='[[1t2l]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[1t2l]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T2L FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2l OCA], [http://pdbe.org/1t2l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t2l RCSB], [http://www.ebi.ac.uk/pdbsum/1t2l PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2l OCA], [http://pdbe.org/1t2l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t2l RCSB], [http://www.ebi.ac.uk/pdbsum/1t2l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t2l ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 12: / Line 13: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t2/1t2l_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t2/1t2l_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t2l ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">

1t2l

From Proteopedia

Revision as of 07:49, 14 March 2018

Three Crystal Structures of Human Coactosin-like Protein

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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