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2l6m

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Revision as of 20:08, 1 August 2018

Structure of C-terminal dsRBD of the Fission Yeast DICER (Dcr1)

Structural highlights

2l6m is a 1 chain structure with sequence from Cbs 356. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	dcr1, SPCC188.13c, SPCC584.10c (CBS 356)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[DCR1_SCHPO] Required for G1 arrest and mating in response to nitrogen starvation. Ago1 regulation of cytokinesis and cell cycle checkpoints occurs downstream of dcr1. Required, indirectly, for regulated hyperphosphorylation of cdc2. Has a role in the RNA interference (RNAi) pathway which is important for heterochromatin formation, accurate chromosome segregation, centromere cohesion and telomere function during mitosis and meiosis. Digests double-stranded RNA (dsRNA) producing 21 to 23 bp dsRNAs, so-called interfering RNAs (siRNA). Required for both post-transcriptional and transcriptional gene silencing. Required for silencing at the centromeres and for initiation of transcriptionally silent heterochromatin at the mating type locus. Promotes histone H3 'Lys-10' methylation necessary for centromere function. Required for recruitment of swi6 and cohesin to an ectopic dg repeat.

Publication Abstract from PubMed

Dicer proteins function in RNA interference (RNAi) pathways by generating small RNAs (sRNAs). Here, we report the solution structure of the C-terminal domain of Schizosaccharomyces pombe Dicer (Dcr1). The structure reveals an unusual double-stranded RNA binding domain (dsRBD) fold embedding a novel zinc-binding motif that is conserved among dicers in yeast. Although the C-terminal domain of Dcr1 still binds nucleic acids, this property is dispensable for proper functioning of Dcr1. In contrast, disruption of zinc coordination renders Dcr1 mainly cytoplasmic and leads to remarkable changes in gene expression and loss of heterochromatin assembly. In summary, our results reveal novel insights into the mechanism of nuclear retention of Dcr1 and raise the possibility that this new class of dsRBDs might generally function in nucleocytoplasmic trafficking and not substrate binding. The C-terminal domain of Dcr1 constitutes a novel regulatory module that might represent a potential target for therapeutic intervention with fungal diseases.

An extended dsRBD with a novel zinc-binding motif mediates nuclear retention of fission yeast Dicer.,Barraud P, Emmerth S, Shimada Y, Hotz HR, Allain FH, Buhler M EMBO J. 2011 Aug 16;30(20):4223-35. doi: 10.1038/emboj.2011.300. PMID:21847092^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Barraud P, Emmerth S, Shimada Y, Hotz HR, Allain FH, Buhler M. An extended dsRBD with a novel zinc-binding motif mediates nuclear retention of fission yeast Dicer. EMBO J. 2011 Aug 16;30(20):4223-35. doi: 10.1038/emboj.2011.300. PMID:21847092 doi:10.1038/emboj.2011.300

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2l6m"

@@ Line 1: / Line 1: @@
 ==Structure of C-terminal dsRBD of the Fission Yeast DICER (Dcr1)==
 <StructureSection load='2l6m' size='340' side='right' caption='[[2l6m]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dcr1, SPCC188.13c, SPCC584.10c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l6m OCA], [http://pdbe.org/2l6m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l6m RCSB], [http://www.ebi.ac.uk/pdbsum/2l6m PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l6m OCA], [http://pdbe.org/2l6m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l6m RCSB], [http://www.ebi.ac.uk/pdbsum/2l6m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2l6m ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 </div>
 <div class="pdbe-citations 2l6m" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Dicer|Dicer]]
+*[[Ribonuclease|Ribonuclease]]
 == References ==
 <references/>

2l6m

From Proteopedia

Revision as of 20:08, 1 August 2018

Structure of C-terminal dsRBD of the Fission Yeast DICER (Dcr1)

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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