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4wt3

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Revision as of 17:47, 16 November 2017

The N-terminal domain of Rubisco Accumulation Factor 1 from Arabidopsis thaliana

Structural highlights

4wt3 is a 1 chain structure with sequence from Arath. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	RAF2, At3g04550, F7O18.2 (ARATH)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RAF2_ARATH] Required for assembly or stability of RuBisCO. Acts at a postchaperonin step to fold and/or assemble the large subunit (LS) into RuBisCO (By similarity).

Publication Abstract from PubMed

Biogenesis of the photosynthetic enzyme Rubisco, a complex of eight large (RbcL) and eight small (RbcS) subunits, requires assembly chaperones. Here we analyzed the role of Rubisco accumulation factor1 (Raf1), a dimer of approximately 40-kDa subunits. We find that Raf1 from Synechococcus elongatus acts downstream of chaperonin-assisted RbcL folding by stabilizing RbcL antiparallel dimers for assembly into RbcL8 complexes with four Raf1 dimers bound. Raf1 displacement by RbcS results in holoenzyme formation. Crystal structures show that Raf1 from Arabidopsis thaliana consists of a beta-sheet dimerization domain and a flexibly linked alpha-helical domain. Chemical cross-linking and EM reconstruction indicate that the beta-domains bind along the equator of each RbcL2 unit, and the alpha-helical domains embrace the top and bottom edges of RbcL2. Raf1 fulfills a role similar to that of the assembly chaperone RbcX, thus suggesting that functionally redundant factors ensure efficient Rubisco biogenesis.

Structure and mechanism of the Rubisco-assembly chaperone Raf1.,Hauser T, Bhat JY, Milicic G, Wendler P, Hartl FU, Bracher A, Hayer-Hartl M Nat Struct Mol Biol. 2015 Aug 3. doi: 10.1038/nsmb.3062. PMID:26237510^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hauser T, Bhat JY, Milicic G, Wendler P, Hartl FU, Bracher A, Hayer-Hartl M. Structure and mechanism of the Rubisco-assembly chaperone Raf1. Nat Struct Mol Biol. 2015 Aug 3. doi: 10.1038/nsmb.3062. PMID:26237510 doi:http://dx.doi.org/10.1038/nsmb.3062

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4wt3"

@@ Line 1: / Line 1: @@
 ==The N-terminal domain of Rubisco Accumulation Factor 1 from Arabidopsis thaliana==
 <StructureSection load='4wt3' size='340' side='right' caption='[[4wt3]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4wt3]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WT3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WT3 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4wt3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WT3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WT3 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wt3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wt3 OCA], [http://pdbe.org/4wt3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wt3 RCSB], [http://www.ebi.ac.uk/pdbsum/4wt3 PDBsum]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RAF2, At3g04550, F7O18.2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wt3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wt3 OCA], [http://pdbe.org/4wt3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wt3 RCSB], [http://www.ebi.ac.uk/pdbsum/4wt3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wt3 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 20: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Arath]]
 [[Category: Bhat, J Y]]
 [[Category: Bracher, A]]

4wt3

From Proteopedia

Revision as of 17:47, 16 November 2017

The N-terminal domain of Rubisco Accumulation Factor 1 from Arabidopsis thaliana

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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