5c6k
From Proteopedia
(Difference between revisions)
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| - | ''' | + | ==Bacteriophage P2 integrase catalytic domain== |
| + | <StructureSection load='5c6k' size='340' side='right' caption='[[5c6k]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5c6k]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C6K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C6K FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c6k OCA], [http://pdbe.org/5c6k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c6k RCSB], [http://www.ebi.ac.uk/pdbsum/5c6k PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/VINT_BPP2 VINT_BPP2]] Integrase is necessary for integration of the phage into the host genome by site-specific recombination. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacteriophage P2 is a temperate phage capable of integrating its DNA into the host genome by site-specific recombination upon lysogenization. Integration and excision of the phage genome requires P2 integrase, which performs recognition, cleavage and joining of DNA during these processes. This work presents the high-resolution crystal structure of the catalytic domain of P2 integrase, and analysis of the structure-function relationship of several previously identified non-functional P2 integrase mutants. The DNA binding area is characterized by a large positively charged patch, harboring key residues. The structure reveals potential for large dimer flexibility, likely essential for rearrangement of DNA strands upon integration and excision of the phage DNA. | ||
| - | + | Crystal structure of the bacteriophage P2 integrase catalytic domain.,Skaar K, Claesson M, Odegrip R, Hogbom M, Haggard-Ljungquist E, Stenmark P FEBS Lett. 2015 Oct 8. pii: S0014-5793(15)00875-3. doi:, 10.1016/j.febslet.2015.09.026. PMID:26453836<ref>PMID:26453836</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5c6k" style="background-color:#fffaf0;"></div> | |
| - | [[Category: | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Claesson, M]] | ||
| + | [[Category: Eriksson, J]] | ||
| + | [[Category: Haggard-Ljungquist, E]] | ||
[[Category: Hogbom, M]] | [[Category: Hogbom, M]] | ||
| - | [[Category: Eriksson, J]] | ||
| - | [[Category: Claesson, M]] | ||
| - | [[Category: Stenmark, P]] | ||
[[Category: Odegrip, R]] | [[Category: Odegrip, R]] | ||
| - | [[Category: Haggard-Ljungquist, E]] | ||
[[Category: Skaar, K]] | [[Category: Skaar, K]] | ||
| + | [[Category: Stenmark, P]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Integrase]] | ||
| + | [[Category: Integration]] | ||
| + | [[Category: Site-specific recombination]] | ||
| + | [[Category: Tyrosine recombinase]] | ||
Revision as of 05:23, 22 October 2015
Bacteriophage P2 integrase catalytic domain
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