This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5d92
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | ''' | + | ==Structure of a phosphatidylinositolphosphate (PIP) synthase from Renibacterium Salmoninarum== |
| + | <StructureSection load='5d92' size='340' side='right' caption='[[5d92]], [[Resolution|resolution]] 3.62Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5d92]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D92 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5D92 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=58A:5-O-[(R)-{[(S)-{(2R)-2,3-BIS[(9E)-OCTADEC-9-ENOYLOXY]PROPOXY}(HYDROXY)PHOSPHORYL]OXY}(HYDROXY)PHOSPHORYL]CYTIDINE'>58A</scene>, <scene name='pdbligand=8K6:OCTADECANE'>8K6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5d91|5d91]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d92 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d92 OCA], [http://pdbe.org/5d92 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d92 RCSB], [http://www.ebi.ac.uk/pdbsum/5d92 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Phosphatidylinositol is critical for intracellular signalling and anchoring of carbohydrates and proteins to outer cellular membranes. The defining step in phosphatidylinositol biosynthesis is catalysed by CDP-alcohol phosphotransferases, transmembrane enzymes that use CDP-diacylglycerol as donor substrate for this reaction, and either inositol in eukaryotes or inositol phosphate in prokaryotes as the acceptor alcohol. Here we report the structures of a related enzyme, the phosphatidylinositol-phosphate synthase from Renibacterium salmoninarum, with and without bound CDP-diacylglycerol to 3.6 and 2.5 A resolution, respectively. These structures reveal the location of the acceptor site, and the molecular determinants of substrate specificity and catalysis. Functional characterization of the 40%-identical ortholog from Mycobacterium tuberculosis, a potential target for the development of novel anti-tuberculosis drugs, supports the proposed mechanism of substrate binding and catalysis. This work therefore provides a structural and functional framework to understand the mechanism of phosphatidylinositol-phosphate biosynthesis. | ||
| - | + | Structural basis for phosphatidylinositol-phosphate biosynthesis.,Clarke OB, Tomasek D, Jorge CD, Dufrisne MB, Kim M, Banerjee S, Rajashankar KR, Shapiro L, Hendrickson WA, Santos H, Mancia F Nat Commun. 2015 Oct 16;6:8505. doi: 10.1038/ncomms9505. PMID:26510127<ref>PMID:26510127</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5d92" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
[[Category: Banerjee, S]] | [[Category: Banerjee, S]] | ||
| - | [[Category: | + | [[Category: Clarke, O B]] |
| - | [[Category: | + | [[Category: Dufrisne, M Belcher]] |
| - | [[Category: Hendrickson, W | + | [[Category: Hendrickson, W A]] |
| + | [[Category: Jorge, C D]] | ||
[[Category: Kim, M]] | [[Category: Kim, M]] | ||
[[Category: Mancia, F]] | [[Category: Mancia, F]] | ||
| - | [[Category: | + | [[Category: Rajashankar, K R]] |
| + | [[Category: Santos, H]] | ||
| + | [[Category: Tomasek, D T]] | ||
| + | [[Category: Enzyme]] | ||
| + | [[Category: Lipid biosynthesis]] | ||
| + | [[Category: Membrane protein]] | ||
| + | [[Category: Phosphatidylinositol]] | ||
Revision as of 20:56, 30 November 2015
Structure of a phosphatidylinositolphosphate (PIP) synthase from Renibacterium Salmoninarum
| |||||||||||
