Cas9 Sandbox
From Proteopedia
(New page: ==Your Heading Here (maybe something like 'Structure')== <StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''> This is a default text for you...) |
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| - | + | [[Cas9]] is the RNA-guided [[DNA]] [[endonuclease]] used by the CRISPR (clustered regularly interspaced short palindromic repeats)-associated systems to generate double-strand DNA breaks in the invading DNA during an adaptive bacterial immune response. | |
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<Structure load='Insert PDB code or filename here' size='350' frame='true' align='right' caption='Insert caption here' scene='Insert optional scene name here' /> | <Structure load='Insert PDB code or filename here' size='350' frame='true' align='right' caption='Insert caption here' scene='Insert optional scene name here' /> | ||
| - | + | The CRISPR-associated endonuclease [[Cas9]] has been exploited for use in genome editing systems. In such systems, an engineered single-guide RNA (sgRNA) is used to target double-stranded breaks in genomic DNA. Depending on what repair pathway is triggered, often dictated by the inclusion of additional engineered components, the targeted site either is disrupted or incorporates additional genetic sequences. | |
| - | == | + | [[Image:Layout for schematic and structure with structure.png|660px]] |
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| + | Articles in Proteopedia concerning Cas9 include: | ||
| + | * any? | ||
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| + | ==3D structures of Cas9== | ||
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| + | ===''Streptococcus pyogenes'' Cas9=== | ||
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| + | * [[4un3]], [[4un4]], and [[4un5]] - ''S. pyogenes'' Cas9 bound to sgRNA and target DNA | ||
| + | [[Image:4un3 labeled.png|right|390px]] | ||
| + | * [[4oo8]] - ''S. pyogenes'' Cas9 bound to sgRNA and target DNA | ||
| + | * [[4cmp]] | ||
| + | * [[4cmq]] - Mn<sup>2+</sup>-bound ''S. pyogenes'' Cas9 | ||
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| + | === ''Actinomyces naeslundii'' Cas9=== | ||
| + | *[[4oge]] | ||
| + | *[[4ogc]] - Mn<sup>2+</sup>-bound ''A.s naeslundii'' Cas9 | ||
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| + | ==See Also== | ||
| + | [[HNH endonuclease]] <br/> | ||
| - | == Structural highlights == | ||
| - | This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | ||
| - | </StructureSection> | ||
== References == | == References == | ||
<references/> | <references/> | ||
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| + | [[Category: Crispr]] | ||
| + | [[Category: Crispr-associated]] | ||
| + | [[Category: endonuclease]] | ||
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| + | The CRISPR-associated endonuclease [[Cas9]] has been exploited for use in genome editing systems. In such systems, an engineered single-guide RNA (sgRNA) is used to target double-stranded breaks in genomic DNA. Depending on what repair pathway is triggered, often dictated by the inclusion of additional engineered components, the targeted site either is disrupted or incorporates additional genetic sequences. | ||
| + | |||
| + | [[Image:Layout for schematic and structure with structure.png|660px]] | ||
| + | |||
| + | Articles in Proteopedia concerning Cas9 include: | ||
| + | * any? | ||
| + | |||
| + | ==3D structures of Cas9== | ||
| + | |||
| + | ===''Streptococcus pyogenes'' Cas9=== | ||
| + | |||
| + | * [[4un3]], [[4un4]], and [[4un5]] - ''S. pyogenes'' Cas9 bound to sgRNA and target DNA | ||
| + | [[Image:4un3 labeled.png|right|390px]] | ||
| + | * [[4oo8]] - ''S. pyogenes'' Cas9 bound to sgRNA and target DNA | ||
| + | * [[4cmp]] | ||
| + | * [[4cmq]] - Mn<sup>2+</sup>-bound ''S. pyogenes'' Cas9 | ||
| + | |||
| + | === ''Actinomyces naeslundii'' Cas9=== | ||
| + | *[[4oge]] | ||
| + | *[[4ogc]] - Mn<sup>2+</sup>-bound ''A.s naeslundii'' Cas9 | ||
| + | |||
| + | ==See Also== | ||
| + | [[HNH endonuclease]] <br/> | ||
| + | |||
| + | |||
| + | |||
| + | == References == | ||
| + | <references/> | ||
| + | |||
| + | [[Category: Crispr]] | ||
| + | [[Category: Crispr-associated]] | ||
| + | [[Category: endonuclease]] | ||
Revision as of 12:42, 6 October 2015
Cas9 is the RNA-guided DNA endonuclease used by the CRISPR (clustered regularly interspaced short palindromic repeats)-associated systems to generate double-strand DNA breaks in the invading DNA during an adaptive bacterial immune response.
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The CRISPR-associated endonuclease Cas9 has been exploited for use in genome editing systems. In such systems, an engineered single-guide RNA (sgRNA) is used to target double-stranded breaks in genomic DNA. Depending on what repair pathway is triggered, often dictated by the inclusion of additional engineered components, the targeted site either is disrupted or incorporates additional genetic sequences.
Articles in Proteopedia concerning Cas9 include:
- any?
Contents |
3D structures of Cas9
Streptococcus pyogenes Cas9
Actinomyces naeslundii Cas9
See Also
References
The CRISPR-associated endonuclease Cas9 has been exploited for use in genome editing systems. In such systems, an engineered single-guide RNA (sgRNA) is used to target double-stranded breaks in genomic DNA. Depending on what repair pathway is triggered, often dictated by the inclusion of additional engineered components, the targeted site either is disrupted or incorporates additional genetic sequences.
Articles in Proteopedia concerning Cas9 include:
- any?
3D structures of Cas9
Streptococcus pyogenes Cas9
Actinomyces naeslundii Cas9
See Also
