5cpg

Publication Abstract from PubMed

(R)-Specific enoyl-coenzyme A (enoyl-CoA) hydratases (PhaJs) are capable of supplying monomers from fatty acid beta-oxidation to polyhydroxyalkanoate (PHA) biosynthesis. Previous study suggested that despite sharing 67% amino acid sequence identity, PhaJ1Pp from Pseudomonas putida showed broader substrate specificity than did PhaJ1Pa from Pseudomonas aeruginosa. In this study, the substrate specificity characteristics of two Pseudomonas PhaJ1 enzymes were investigated by site-directed mutagenesis, chimeragenesis, X-ray crystallographic analysis, and homology modeling. In PhaJ1Pp, the substitution of valine with isoleucine at position 72 resulted in an increased preference for enoyl-CoAs with shorter chain lengths. Conversely, at the same position in PhaJ1Pa, the substitution of isoleucine with valine resulted in an increased preference for enoyl-CoAs with longer chain lengths. These changes suggest a narrowing and broadening in the substrate specificity range of the PhaJ1Pp and PhaJ1Pa mutants, respectively. However, the substrate specificity remains broader in PhaJ1Pp than in PhaJ1Pa. Additionally, three chimeric PhaJ1 enzymes, composed from PhaJ1Pp and PhaJ1Pa, all showed significant hydratase activity, and their substrate preferences were within the range exhibited by the parental PhaJ1 enzymes. The crystal structure of PhaJ1Pa was determined at a resolution of 1.7 A and subsequent homology modeling of PhaJ1Pp revealed that in the acyl-chain binding pocket, the amino acid at position 72 was the only difference between the two structures. These results indicate that the chain-length specificity of PhaJ1 is determined mainly by the bulkiness of the amino acid residue at position 72, but that other factors such as structural fluctuations may also affect specificity.

Contribution of the distal pocket residue to the acyl-chain-length substrate specificity of (R)-specific enoyl-CoA hydratases from Pseudomonas: site-directed mutagenesis, chimeragenesis, X-ray crystallographic analysis, and homology modeling.,Tsuge T, Sato S, Hiroe A, Ishizuka K, Kanazawa H, Shiro Y, Hisano T Appl Environ Microbiol. 2015 Sep 18. pii: AEM.02412-15. PMID:26386053^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.