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5dbj
From Proteopedia
(Difference between revisions)
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dbj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dbj OCA], [http://pdbe.org/5dbj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dbj RCSB], [http://www.ebi.ac.uk/pdbsum/5dbj PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dbj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dbj OCA], [http://pdbe.org/5dbj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dbj RCSB], [http://www.ebi.ac.uk/pdbsum/5dbj PDBsum]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Pyoluteorin is an antifungal agent composed of a 4,5-dichlorinated pyrrole group linked to a resorcinol moiety. The pyoluteorin biosynthetic gene cluster in Pseudomonas fluorescens Pf-5 encodes the halogenase PltA, which has been previously demonstrated to perform both chlorinations in vitro. PltA selectively accepts as a substrate a pyrrole moiety covalently tethered to a nonribosomal peptide thiolation domain PltL (pyrrolyl-S-PltL) for FAD-dependent di-chlorination, yielding 4,5-dichloropyrrolyl-S-PltL. We report a 2.75A-resolution crystal structure of PltA in complex with FAD and chloride. PltA is a dimeric enzyme, containing a flavin-binding fold conserved in flavin-dependent halogenases and monooxygenases, and an additional unique helical region at the C-terminus. This C-terminal region blocks a putative substrate-binding cleft, suggesting that a conformational change involving repositioning of this region is necessary to allow binding of the pyrrolyl-S-PltL substrate for its dichlorination by PltA. | ||
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| + | Crystal structure of halogenase PltA from the pyoluteorin biosynthetic pathway.,Pang AH, Garneau-Tsodikova S, Tsodikov OV J Struct Biol. 2015 Sep 28. pii: S1047-8477(15)30068-X. doi:, 10.1016/j.jsb.2015.09.013. PMID:26416533<ref>PMID:26416533</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5dbj" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 21:45, 15 October 2015
Crystal structure of halogenase PltA
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