DNA polymerase
From Proteopedia
| Line 1: | Line 1: | ||
| - | {{STRUCTURE_1taq| PDB=1taq | SIZE=350| SCENE=DNA_polymerase/Cv/1 |right|CAPTION=DNA polymerase complex with octylglucoside [[1taq]] }} | + | {{STRUCTURE_1taq| PDB=1taq | SIZE=350| SCENE=DNA_polymerase/Cv/1 |right|CAPTION=Family A DNA polymerase complex with octylglucoside [[1taq]] }} |
[[DNA polymerase]] '''(Dpo)''' reads a [[DNA|DNA strand]] and catalyzes the synthesis of its complementary strand. Dpo's are divided into 7 families according to their sequence homology and 3D structure similarities. The families are:<br /> | [[DNA polymerase]] '''(Dpo)''' reads a [[DNA|DNA strand]] and catalyzes the synthesis of its complementary strand. Dpo's are divided into 7 families according to their sequence homology and 3D structure similarities. The families are:<br /> | ||
| Line 43: | Line 43: | ||
**[[1d9d]], [[1d9f]], [[2kfn]] - EcDpo I klenow fragment + DNA + nucleotide<br /> | **[[1d9d]], [[1d9f]], [[2kfn]] - EcDpo I klenow fragment + DNA + nucleotide<br /> | ||
**[[2kfz]] - EcDpo I klenow fragment (mutant) + DNA + nucleotide<br /> | **[[2kfz]] - EcDpo I klenow fragment (mutant) + DNA + nucleotide<br /> | ||
| - | **[[1taq]] – TaDpo I - ''Thermus aquaticus''<br /> | + | **[[1taq]] – TaDpo I (mutant) - ''Thermus aquaticus''<br /> |
**[[1jxe]] – TaDpo I Stoffel fragment<br /> | **[[1jxe]] – TaDpo I Stoffel fragment<br /> | ||
**[[1ktq]] - TaDpo I klenow fragment<br /> | **[[1ktq]] - TaDpo I klenow fragment<br /> | ||
Revision as of 09:02, 14 October 2015
DNA polymerase (Dpo) reads a DNA strand and catalyzes the synthesis of its complementary strand. Dpo's are divided into 7 families according to their sequence homology and 3D structure similarities. The families are:
- Family A - replicative or repair Dpo.
- Family B - replicative Dpo involved in processing DNA replication during cell division (includes eukaryotic Dpo α,δ,ε).
- Family C - major replicative Dpo in bacteria (includes Dpo II, III, IV).
- Family D - replicative. Present in archaea.
- Family X - eukaryotic repair Dpo (includes Dpo β,λ,σ,μ and terminal deoxynucleotidyl transferase).
- Family Y - replicative of damaged DNA (includes eukaryotic η,ι,κ).
- Family RT - reverse transcriptase. See Reverse transcriptase.
Some Dpo terminology:
Dpo sliding clamp is made of the complex of Dpo and Proliferating Cell Nuclear Antigen (PCNA) which encircles it.
The BRCT domain in Dpo is the C-terminal domain of breast cancer susceptibility protein.
Klenow fragment is a large Dpo fragment produced upon cleavage of Dpo by subtilisin.
In the E. coli, the EcDpo III subunits β, γ, δ, δ' are named clamp loader. This complex assembles the β subunit sliding clamp unto the DNA.
Rev1 is a Dpo involved in replication over DNA lesions.
Terminal deoxynucleotidyl transferase (TdT) is a family X Dpo expressed in immature lymphoid cells. TdT adds nucleotides to exons during antibody gene recombination.
For more details see
- DNA Polymerase I
- Polymerase III homoenzyme beta subunit
- User:Karl E. Zahn/RB69 DNA polymerase (gp43)
- Alpha Subunit of Thermus aquaticus DNA Polymerase III.
3D Structures of DNA polymerase
Updated on 14-October-2015
Proteopedia Page Contributors and Editors (what is this?)
Michal Harel, Cori Damron, Alexander Berchansky, Jaime Prilusky, Emily Bray, Joel L. Sussman, David Canner
