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4yfz

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Revision as of 06:14, 6 October 2017

Structural basis of glycan recognition in neonate-specific rotaviruses

Structural highlights

4yfz is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	4yfw, 4ye2, 4yg0, 4yg3, 4yg6
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[B6RGK2_9REOV] Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm. In integrin-dependent strains, VP5* targets the integrin heterodimer ITGA2/ITGB1 for cell attachment (By similarity).[SAAS:SAAS00209906] VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies. In sialic acid-dependent strains, VP8* binds to host cell sialic acid, most probably a ganglioside, providing the initial contact (By similarity).[SAAS:SAAS00209911]

Publication Abstract from PubMed

Strain-dependent variation of glycan recognition during initial cell attachment of viruses is a critical determinant of host specificity, tissue-tropism and zoonosis. Rotaviruses (RVs), which cause life-threatening gastroenteritis in infants and children, display significant genotype-dependent variations in glycan recognition resulting from sequence alterations in the VP8* domain of the spike protein VP4. The structural basis of this genotype-dependent glycan specificity, particularly in human RVs, remains poorly understood. Here, from crystallographic studies, we show how genotypic variations configure a novel binding site in the VP8* of a neonate-specific bovine-human reassortant to uniquely recognize either type I or type II precursor glycans, and to restrict type II glycan binding in the bovine counterpart. Such a distinct glycan-binding site that allows differential recognition of the precursor glycans, which are developmentally regulated in the neonate gut and abundant in bovine and human milk provides a basis for age-restricted tropism and zoonotic transmission of G10P[11] rotaviruses.

Structural basis of glycan specificity in neonate-specific bovine-human reassortant rotavirus.,Hu L, Ramani S, Czako R, Sankaran B, Yu Y, Smith DF, Cummings RD, Estes MK, Venkataram Prasad BV Nat Commun. 2015 Sep 30;6:8346. doi: 10.1038/ncomms9346. PMID:26420502^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hu L, Ramani S, Czako R, Sankaran B, Yu Y, Smith DF, Cummings RD, Estes MK, Venkataram Prasad BV. Structural basis of glycan specificity in neonate-specific bovine-human reassortant rotavirus. Nat Commun. 2015 Sep 30;6:8346. doi: 10.1038/ncomms9346. PMID:26420502 doi:http://dx.doi.org/10.1038/ncomms9346

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4yfz"

@@ Line 1: / Line 1: @@
 ==Structural basis of glycan recognition in neonate-specific rotaviruses==
 <StructureSection load='4yfz' size='340' side='right' caption='[[4yfz]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4yfw|4yfw]], [[4ye2|4ye2]], [[4yg0|4yg0]], [[4yg3|4yg3]], [[4yg6|4yg6]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yfz OCA], [http://pdbe.org/4yfz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yfz RCSB], [http://www.ebi.ac.uk/pdbsum/4yfz PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yfz OCA], [http://pdbe.org/4yfz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yfz RCSB], [http://www.ebi.ac.uk/pdbsum/4yfz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4yfz ProSAT]</span></td></tr>
 </table>
 == Function ==

4yfz

From Proteopedia

Revision as of 06:14, 6 October 2017

Structural basis of glycan recognition in neonate-specific rotaviruses

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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