This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5fip
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | ''' | + | ==Discovery and characterization of a novel thermostable and highly halotolerant GH5 cellulase from an Icelandic hot spring isolate== |
| + | <StructureSection load='5fip' size='340' side='right' caption='[[5fip]], [[Resolution|resolution]] 1.88Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5fip]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Unidentified Unidentified]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FIP FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PG6:1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE'>PG6</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fip OCA], [http://pdbe.org/5fip PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fip RCSB], [http://www.ebi.ac.uk/pdbsum/5fip PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | With the ultimate goal of identifying robust cellulases for industrial biocatalytic conversions, we have isolated and characterized a new thermostable and very halotolerant GH5 cellulase. This new enzyme, termed CelDZ1, was identified by bioinformatic analysis from the genome of a polysaccharide-enrichment culture isolate, initiated from material collected from an Icelandic hot spring. Biochemical characterization of CelDZ1 revealed that it is a glycoside hydrolase with optimal activity at 70 degrees C and pH 5.0 that exhibits good thermostability, high halotolerance at near-saturating salt concentrations, and resistance towards metal ions and other denaturing agents. X-ray crystallography of the new enzyme showed that CelDZ1 is the first reported cellulase structure that lacks the defined sugar-binding 2 subsite and revealed structural features which provide potential explanations of its biochemical characteristics. | ||
| - | + | Discovery and Characterization of a Thermostable and Highly Halotolerant GH5 Cellulase from an Icelandic Hot Spring Isolate.,Zarafeta D, Kissas D, Sayer C, Gudbergsdottir SR, Ladoukakis E, Isupov MN, Chatziioannou A, Peng X, Littlechild JA, Skretas G, Kolisis FN PLoS One. 2016 Jan 7;11(1):e0146454. doi: 10.1371/journal.pone.0146454., eCollection 2016. PMID:26741138<ref>PMID:26741138</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5fip" style="background-color:#fffaf0;"></div> | |
| - | [[Category: | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Cellulase]] | ||
| + | [[Category: Unidentified]] | ||
| + | [[Category: Chatziioannou, A]] | ||
| + | [[Category: Gudbergsdottir, S R]] | ||
| + | [[Category: Isupov, M N]] | ||
| + | [[Category: Kissas, D]] | ||
| + | [[Category: Kolisis, F N]] | ||
[[Category: Ladoukakis, E]] | [[Category: Ladoukakis, E]] | ||
| + | [[Category: Littlechild, J A]] | ||
[[Category: Peng, X]] | [[Category: Peng, X]] | ||
| - | [[Category: | + | [[Category: Sayer, C]] |
[[Category: Skretas, G]] | [[Category: Skretas, G]] | ||
| - | [[Category: Gudbergsdottir, S.R]] | ||
| - | [[Category: Kolisis, F.N]] | ||
| - | [[Category: Chatziioannou, A]] | ||
[[Category: Zarafeta, D]] | [[Category: Zarafeta, D]] | ||
| - | [[Category: | + | [[Category: Gh5]] |
| - | [[Category: | + | [[Category: Hydrolase]] |
| - | + | ||
Revision as of 16:57, 20 January 2016
Discovery and characterization of a novel thermostable and highly halotolerant GH5 cellulase from an Icelandic hot spring isolate
| |||||||||||
Categories: Cellulase | Unidentified | Chatziioannou, A | Gudbergsdottir, S R | Isupov, M N | Kissas, D | Kolisis, F N | Ladoukakis, E | Littlechild, J A | Peng, X | Sayer, C | Skretas, G | Zarafeta, D | Gh5 | Hydrolase
