Tyrone Evans Hox Proteins sandbox
From Proteopedia
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Hox Proteins are transcription factors, proteins that are involved in the process of transcribing DNA into RNA. These specific types of proteins are capable of binding to enhancers, a short (50-1500 bp) region of DNA, in pursuit of activating or repressing certain genes. These proteins are named this way because of the mutations that are done within them that cause homeotic transformations, changing of an organ into another. | Hox Proteins are transcription factors, proteins that are involved in the process of transcribing DNA into RNA. These specific types of proteins are capable of binding to enhancers, a short (50-1500 bp) region of DNA, in pursuit of activating or repressing certain genes. These proteins are named this way because of the mutations that are done within them that cause homeotic transformations, changing of an organ into another. | ||
- | == Role in Development | + | == Role in Development == |
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- | As mentioned previously, homeotic transformations can alter DNA sequences so that specific proteins will have different functions, and certain genes will be either activated or deactivated. More specifically, if a certain hox protein is responsible for the development of legs on a fly in the middle region of its body, a homeotic transformation can cause this protein to carry out this same function in a different area. In this example, this altered protein can activate the gene for leg development to change the antennae to legs on that fly's head, as seen in multiple experiments. And biologists believe this to be true for Hox genes in flies, mice, humans, and other animals. | + | As mentioned previously, homeotic transformations can alter DNA sequences so that specific proteins will have different functions, and certain genes will be either activated or deactivated. More specifically, if a certain hox protein is responsible for the development of legs on a fly in the middle region of its body, a homeotic transformation can cause this protein to carry out this same function in a different area. In this example, this altered protein can activate the gene for leg development to change the antennae to legs on that fly's head, as seen in multiple experiments. And biologists believe this to be true for Hox genes in flies, mice, humans, and other animals<ref>Freeman, Quillin, and Allison. Biological Science. 5th ed. Vol. 1. N.p.: Benjamin Cummings, n.d. Print. |
+ | </ref>. | ||
== Hox Protein Regulation == | == Hox Protein Regulation == | ||
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== Interaction with DNA == | == Interaction with DNA == | ||
- | A homeodomain is an essential part that all Hox proteins possess. The homeodomain can be found within the homeobox, a DNA sequence found within genes that are responsible of morphogenesis of living organisms. This sequence codes for homeodomain protein products, 60 amino-acid segments, which have specific folding patterns (helix-turn-helix) that allows them to bind with DNA through a <scene name='71/714951/Taaat_sequence/1'>5’-TAAAT-3’</scene> core motif. This domain consist of three helical regions folded into a tight spherical structure (need picture of structure). There are two antiparallel N-terminal helices and one C-terminal helix within this domain. The C-terminal helix binds directly with DNA through the use of <scene name='71/714951/H_bond_between_gln_and_p/1'>Hydrogen bonds</scene> and <scene name='71/714951/Hydrophobic_interaction/3'>Hydrophobic interactions</scene> between side chains and the outer bases and thymine methyl groups within the major groove of a DNA helix. Ionic bonding can also be observed between the bases of the DNA sequence and the amino acids of the protein dimers. A more specific <scene name='71/714951/Ionic_bond/1'>Ionic interaction</scene> is the one between LYS 207 and an oxygen atom of a phosphate group. The <scene name='71/714951/Recognition_helix/1'>Recognition helix</scene> within homeodomain binds within the <scene name='71/714951/Dna_major_groove/1'>major groove</scene> of a DNA helix, while the <scene name='71/714951/Amino-terminal_tail/1'>Amino-Terminal tail</scene> binds within the <scene name='71/714951/Dna_minor_groove/1'>minor groove</scene> of a DNA helix. | + | A homeodomain is an essential part that all Hox proteins possess. The homeodomain can be found within the homeobox, a DNA sequence found within genes that are responsible of morphogenesis of living organisms. This sequence codes for homeodomain protein products, 60 amino-acid segments, which have specific folding patterns (helix-turn-helix) that allows them to bind with DNA through a <scene name='71/714951/Taaat_sequence/1'>5’-TAAAT-3’</scene> core motif. This domain consist of three helical regions folded into a tight spherical structure (need picture of structure). There are two antiparallel N-terminal helices and one C-terminal helix within this domain. The C-terminal helix binds directly with DNA through the use of <scene name='71/714951/H_bond_between_gln_and_p/1'>Hydrogen bonds</scene> and <scene name='71/714951/Hydrophobic_interaction/3'>Hydrophobic interactions</scene> between side chains and the outer bases and thymine methyl groups within the major groove of a DNA helix. Ionic bonding can also be observed between the bases of the DNA sequence and the amino acids of the protein dimers. A more specific <scene name='71/714951/Ionic_bond/1'>Ionic interaction</scene> is the one between LYS 207 and an oxygen atom of a phosphate group. The <scene name='71/714951/Recognition_helix/1'>Recognition helix</scene> within homeodomain binds within the <scene name='71/714951/Dna_major_groove/1'>major groove</scene> of a DNA helix, while the <scene name='71/714951/Amino-terminal_tail/1'>Amino-Terminal tail</scene> binds within the <scene name='71/714951/Dna_minor_groove/1'>minor groove</scene> of a DNA helix<ref>Ladam, Franck, and Charles G. Sagerström. “Hox Regulation of Transcription – More Complex(es).” Developmental dynamics : an official publication of the American Association of Anatomists 243.1 (2014): 10.1002/dvdy.23997. PMC. Web. 3 Nov. 2015.</ref>. |
- | == Human Hox Genes == | + | == Human Hox Genes<ref>"Hox Gene." Wikipedia. Wikimedia Foundation, 14 Aug. 2015. Web. 20 Oct. 2015.</ref> == |
The cluster HOX-A corresponds with chromosome 7, regulating genes: HOX-A1, HOX-A2, HOX-A3, HOX-A4, HOX-A5, HOX-A6, HOX-A7, HOX-A9, HOXA-10, HOXA-11, HOXA-13. | The cluster HOX-A corresponds with chromosome 7, regulating genes: HOX-A1, HOX-A2, HOX-A3, HOX-A4, HOX-A5, HOX-A6, HOX-A7, HOX-A9, HOXA-10, HOXA-11, HOXA-13. |
Revision as of 18:09, 3 November 2015
Hox Proteins
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