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5a4h

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Revision as of 17:09, 16 November 2017

Solution structure of the lipid droplet anchoring peptide of CGI-58 bound to DPC micelles

Structural highlights

5a4h is a 1 chain structure with sequence from Lk3 transgenic mice. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Activity:	1-acylglycerol-3-phosphate O-acyltransferase, with EC number 2.3.1.51
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ABHD5_MOUSE] Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation.^[1]

Publication Abstract from PubMed

Triacylglycerols (TGs) stored in lipid droplets (LDs) are hydrolyzed in a highly regulated metabolic process called lipolysis to free fatty acids that serve as energy substrates for beta-oxidation, precursors for membrane lipids and signaling molecules. Comparative gene identification-58 (CGI-58) stimulates the enzymatic activity of adipose triglyceride lipase (ATGL), which catalyzes the hydrolysis of TGs to diacylglycerols and free fatty acids. In adipose tissue, protein-protein interactions between CGI-58 and the LD coating protein perilipin 1 restrain the ability of CGI-58 to activate ATGL under basal conditions. Phosphorylation of perilipin 1 disrupts these interactions and mobilizes CGI-58 for the activation of ATGL. We have previously demonstrated that the removal of a peptide at the N terminus (residues 10-31) of CGI-58 abrogates CGI-58 localization to LDs and CGI-58-mediated activation of ATGL. Here, we show that this tryptophan-rich N-terminal peptide serves as an independent LD anchor, with its three tryptophans serving as focal points of the left (harboring Trp(21) and Trp(25)) and right (harboring Trp(29)) anchor arms. The solution state NMR structure of a peptide comprising the LD anchor bound to dodecylphosphocholine micelles as LD mimic reveals that the left arm forms a concise hydrophobic core comprising tryptophans Trp(21) and Trp(25) and two adjacent leucines. Trp(29) serves as the core of a functionally independent anchor arm. Consequently, simultaneous tryptophan alanine permutations in both arms abolish localization and activity of CGI-58 as opposed to tryptophan substitutions that occur in only one arm.

Structure of a CGI-58 Motif Provides the Molecular Basis of Lipid Droplet Anchoring.,Boeszoermenyi A, Nagy HM, Arthanari H, Pillip CJ, Lindermuth H, Luna RE, Wagner G, Zechner R, Zangger K, Oberer M J Biol Chem. 2015 Oct 30;290(44):26361-72. doi: 10.1074/jbc.M115.682203. Epub, 2015 Sep 8. PMID:26350461^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lass A, Zimmermann R, Haemmerle G, Riederer M, Schoiswohl G, Schweiger M, Kienesberger P, Strauss JG, Gorkiewicz G, Zechner R. Adipose triglyceride lipase-mediated lipolysis of cellular fat stores is activated by CGI-58 and defective in Chanarin-Dorfman Syndrome. Cell Metab. 2006 May;3(5):309-19. PMID:16679289 doi:http://dx.doi.org/10.1016/j.cmet.2006.03.005
↑ Boeszoermenyi A, Nagy HM, Arthanari H, Pillip CJ, Lindermuth H, Luna RE, Wagner G, Zechner R, Zangger K, Oberer M. Structure of a CGI-58 Motif Provides the Molecular Basis of Lipid Droplet Anchoring. J Biol Chem. 2015 Oct 30;290(44):26361-72. doi: 10.1074/jbc.M115.682203. Epub, 2015 Sep 8. PMID:26350461 doi:http://dx.doi.org/10.1074/jbc.M115.682203

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5a4h"

@@ Line 1: / Line 1: @@
 ==Solution structure of the lipid droplet anchoring peptide of CGI-58 bound to DPC micelles==
 <StructureSection load='5a4h' size='340' side='right' caption='[[5a4h]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5a4h]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A4H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A4H FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5a4h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A4H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A4H FirstGlance]. <br>
 </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-acylglycerol-3-phosphate_O-acyltransferase 1-acylglycerol-3-phosphate O-acyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.51 2.3.1.51] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a4h OCA], [http://pdbe.org/5a4h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a4h RCSB], [http://www.ebi.ac.uk/pdbsum/5a4h PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a4h OCA], [http://pdbe.org/5a4h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a4h RCSB], [http://www.ebi.ac.uk/pdbsum/5a4h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5a4h ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 23: @@
 </StructureSection>
 [[Category: 1-acylglycerol-3-phosphate O-acyltransferase]]
+[[Category: Lk3 transgenic mice]]
 [[Category: Arthanari, H]]
 [[Category: Boeszoermenyi, A]]

5a4h

From Proteopedia

Revision as of 17:09, 16 November 2017

Solution structure of the lipid droplet anchoring peptide of CGI-58 bound to DPC micelles

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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