153d
From Proteopedia
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| - | + | {{STRUCTURE_153d| PDB=153d | SCENE= }} | |
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'''CRYSTAL STRUCTURE OF A MISPAIRED DODECAMER, D(CGAGAATTC(O6ME)GCG)2, CONTAINING A CARCINOGENIC O6-METHYLGUANINE''' | '''CRYSTAL STRUCTURE OF A MISPAIRED DODECAMER, D(CGAGAATTC(O6ME)GCG)2, CONTAINING A CARCINOGENIC O6-METHYLGUANINE''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=153D OCA]. | |
==Reference== | ==Reference== | ||
Crystal structure of a mispaired dodecamer, d(CGAGAATTC(O6Me)GCG)2, containing a carcinogenic O6-methylguanine., Ginell SL, Vojtechovsky J, Gaffney B, Jones R, Berman HM, Biochemistry. 1994 Mar 29;33(12):3487-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8142345 8142345] | Crystal structure of a mispaired dodecamer, d(CGAGAATTC(O6Me)GCG)2, containing a carcinogenic O6-methylguanine., Ginell SL, Vojtechovsky J, Gaffney B, Jones R, Berman HM, Biochemistry. 1994 Mar 29;33(12):3487-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8142345 8142345] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Berman, H M.]] | [[Category: Berman, H M.]] | ||
[[Category: Gaffney, B.]] | [[Category: Gaffney, B.]] | ||
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[[Category: Jones, R.]] | [[Category: Jones, R.]] | ||
[[Category: Vojtechovsky, J.]] | [[Category: Vojtechovsky, J.]] | ||
| - | [[Category: | + | [[Category: B-dna]] |
| - | [[Category: | + | [[Category: Double helix]] |
| - | [[Category: | + | [[Category: Mismatched]] |
| - | [[Category: | + | [[Category: Modified]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:31:00 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:31, 2 May 2008
CRYSTAL STRUCTURE OF A MISPAIRED DODECAMER, D(CGAGAATTC(O6ME)GCG)2, CONTAINING A CARCINOGENIC O6-METHYLGUANINE
Overview
The crystal structure of the synthetic deoxydodecamer d(CGAGAATTC(O6Me)GCG)2 has been determined and refined to an R-factor of 16.9% with data up to 2.9-A resolution. This sequence contains two mismatched base pairs between O6-methylguanine and adenine with the arrangement A(syn).(O6-Me)G(anti) which differs from the geometry observed in solution by NMR. The intermolecular arrangement is equivalent to the other isomorphous deoxydodecamers. However, the weakening of some significant crystal packing contacts was observed and related to the effect of stacking between the mispaired adenine and the adjacent guanine in the sequence. The structure is highly hydrated, with a total of 49 solvent molecules located. The methyl group and the mismatched base-pair geometry locally disrupt the B-DNA-type solvent network with two solvent molecules found close to the N1 and N6 of the mispaired adenine.
About this Structure
Full crystallographic information is available from OCA.
Reference
Crystal structure of a mispaired dodecamer, d(CGAGAATTC(O6Me)GCG)2, containing a carcinogenic O6-methylguanine., Ginell SL, Vojtechovsky J, Gaffney B, Jones R, Berman HM, Biochemistry. 1994 Mar 29;33(12):3487-93. PMID:8142345 Page seeded by OCA on Fri May 2 09:31:00 2008
