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1a0p
From Proteopedia
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'''SITE-SPECIFIC RECOMBINASE, XERD''' | '''SITE-SPECIFIC RECOMBINASE, XERD''' | ||
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[[Category: Subramanya, H S.]] | [[Category: Subramanya, H S.]] | ||
[[Category: Wigley, D B.]] | [[Category: Wigley, D B.]] | ||
| - | [[Category: | + | [[Category: Dna binding]] |
| - | [[Category: | + | [[Category: Dna recombination]] |
| - | [[Category: | + | [[Category: Recombinase]] |
| - | [[Category: | + | [[Category: Xerd]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:38:20 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:38, 2 May 2008
SITE-SPECIFIC RECOMBINASE, XERD
Overview
The structure of the site-specific recombinase, XerD, that functions in circular chromosome separation, has been solved at 2.5 A resolution and reveals that the protein comprises two domains. The C-terminal domain contains two conserved sequence motifs that are located in similar positions in the structures of XerD, lambda and HP1 integrases. However, the extreme C-terminal regions of the three proteins, containing the active site tyrosine, are very different. In XerD, the arrangement of active site residues supports a cis cleavage mechanism. Biochemical evidence for DNA bending is encompassed in a model that accommodates extensive biochemical and genetic data, and in which the DNA is wrapped around an alpha-helix in a manner similar to that observed for CAP complexed with DNA.
About this Structure
1A0P is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of the site-specific recombinase, XerD., Subramanya HS, Arciszewska LK, Baker RA, Bird LE, Sherratt DJ, Wigley DB, EMBO J. 1997 Sep 1;16(17):5178-87. PMID:9311978 Page seeded by OCA on Fri May 2 09:38:20 2008
