5ecj
From Proteopedia
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| - | ''' | + | ==Crystal structure of monobody Mb(S4) bound to Prdm14 in complex with Mtgr1== |
| + | <StructureSection load='5ecj' size='340' side='right' caption='[[5ecj]], [[Resolution|resolution]] 3.05Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5ecj]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ECJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ECJ FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ecj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ecj OCA], [http://pdbe.org/5ecj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ecj RCSB], [http://www.ebi.ac.uk/pdbsum/5ecj PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/PRD14_MOUSE PRD14_MOUSE]] Transcription factor that has both positive and negative roles on transcription. Plays a role in cellular pluripotency (By similarity). Essential for germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression.<ref>PMID:18622394</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Prdm14 is a sequence-specific transcriptional regulator of embryonic stem cell (ESC) pluripotency and primordial germ cell (PGC) formation. It exerts its function, at least in part, through repressing genes associated with epigenetic modification and cell differentiation. Here, we show that this repressive function is mediated through an ETO-family co-repressor Mtgr1, which tightly binds to the pre-SET/SET domains of Prdm14 and co-occupies its genomic targets in mouse ESCs. We generated two monobodies, synthetic binding proteins, targeting the Prdm14 SET domain and demonstrate their utility, respectively, in facilitating crystallization and structure determination of the Prdm14-Mtgr1 complex, or as genetically encoded inhibitor of the Prdm14-Mtgr1 interaction. Structure-guided point mutants and the monobody abrogated the Prdm14-Mtgr1 association and disrupted Prdm14's function in mESC gene expression and PGC formation in vitro. Altogether, our work uncovers the molecular mechanism underlying Prdm14-mediated repression and provides renewable reagents for studying and controlling Prdm14 functions. | ||
| - | + | ETO family protein Mtgr1 mediates Prdm14 functions in stem cell maintenance and primordial germ cell formation.,Nady N, Gupta A, Ma Z, Swigut T, Koide A, Koide S, Wysocka J Elife. 2015 Nov 2;4. pii: e10150. doi: 10.7554/eLife.10150. PMID:26523391<ref>PMID:26523391</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5ecj" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Gupta, A]] | [[Category: Gupta, A]] | ||
[[Category: Koide, S]] | [[Category: Koide, S]] | ||
| + | [[Category: Gene regulation-transcription complex]] | ||
| + | [[Category: Protein binding]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 16:10, 2 December 2015
Crystal structure of monobody Mb(S4) bound to Prdm14 in complex with Mtgr1
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