1a3g

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[[Image:1a3g.gif|left|200px]]
[[Image:1a3g.gif|left|200px]]
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{{Structure
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|PDB= 1a3g |SIZE=350|CAPTION= <scene name='initialview01'>1a3g</scene>, resolution 2.5&Aring;
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The line below this paragraph, containing "STRUCTURE_1a3g", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=PLP:PYRIDOXAL-5&#39;-PHOSPHATE'>PLP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Branched-chain-amino-acid_transaminase Branched-chain-amino-acid transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.42 2.6.1.42] </span>
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{{STRUCTURE_1a3g| PDB=1a3g | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a3g OCA], [http://www.ebi.ac.uk/pdbsum/1a3g PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1a3g RCSB]</span>
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'''BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE FROM ESCHERICHIA COLI'''
'''BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE FROM ESCHERICHIA COLI'''
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[[Category: Okada, K.]]
[[Category: Okada, K.]]
[[Category: Sato, M.]]
[[Category: Sato, M.]]
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[[Category: aminotransferase]]
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[[Category: Aminotransferase]]
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[[Category: pyridoxal enzyme]]
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[[Category: Pyridoxal enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:45:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:32:29 2008''
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Revision as of 06:45, 2 May 2008

Image:1a3g.gif

Template:STRUCTURE 1a3g

BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE FROM ESCHERICHIA COLI


Overview

The X-ray crystallographic structure of the branched-chain amino acid aminotransferase from Escherichia coli was determined by means of isomorphous replacement using the selenomethionyl enzyme as one of the heavy atom derivatives. The enzyme is a homo hexamer with D3 symmetry, and the polypeptide chain of the subunit is folded into two domains (small and large domains). The coenzyme, pyridoxal 5'-phosphate, resides at the domain interface, its re-face facing toward the protein. The active site structure shows that the following sites can recognize branched-chain amino acids and glutamate as substrates: (1) a hydrophobic core formed by Phe36, Tyr164, Tyr31*, and Val109* for a branched-chain; (2) Arg97 for an acidic side chain of glutamate; and (3) Tyr95 and two main chain NH groups of Thr257 and Ala258 for the alpha-carboxylate of substrates. Although the main chain conformation of the active site is homologous to that of D-amino acid aminotransferase, many of the active site residues are different between them.

About this Structure

1A3G is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Three-dimensional structure of Escherichia coli branched-chain amino acid aminotransferase at 2.5 A resolution., Okada K, Hirotsu K, Sato M, Hayashi H, Kagamiyama H, J Biochem. 1997 Apr;121(4):637-41. PMID:9163511 Page seeded by OCA on Fri May 2 09:45:28 2008

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