1a84

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[[Image:1a84.jpg|left|200px]]
[[Image:1a84.jpg|left|200px]]
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{{Structure
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|PDB= 1a84 |SIZE=350|CAPTION= <scene name='initialview01'>1a84</scene>
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The line below this paragraph, containing "STRUCTURE_1a84", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CPT:CIS-PLATINUM-(NH3)2'>CPT</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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{{STRUCTURE_1a84| PDB=1a84 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a84 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a84 OCA], [http://www.ebi.ac.uk/pdbsum/1a84 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1a84 RCSB]</span>
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'''NMR SOLUTION STRUCTURE OF A DNA DODECAMER DUPLEX CONTAINING A CIS-DIAMMINEPLATINUM(II) D(GPG) INTRASTRAND CROSS-LINK, THE MAJOR ADDUCT OF THE ANTICANCER DRUG CISPLATIN, 1 STRUCTURE'''
'''NMR SOLUTION STRUCTURE OF A DNA DODECAMER DUPLEX CONTAINING A CIS-DIAMMINEPLATINUM(II) D(GPG) INTRASTRAND CROSS-LINK, THE MAJOR ADDUCT OF THE ANTICANCER DRUG CISPLATIN, 1 STRUCTURE'''
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==About this Structure==
==About this Structure==
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1A84 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A84 OCA].
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A84 OCA].
==Reference==
==Reference==
NMR solution structure of a DNA dodecamer duplex containing a cis-diammineplatinum(II) d(GpG) intrastrand cross-link, the major adduct of the anticancer drug cisplatin., Gelasco A, Lippard SJ, Biochemistry. 1998 Jun 30;37(26):9230-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9649303 9649303]
NMR solution structure of a DNA dodecamer duplex containing a cis-diammineplatinum(II) d(GpG) intrastrand cross-link, the major adduct of the anticancer drug cisplatin., Gelasco A, Lippard SJ, Biochemistry. 1998 Jun 30;37(26):9230-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9649303 9649303]
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[[Category: Protein complex]]
 
[[Category: Gelasco, A.]]
[[Category: Gelasco, A.]]
[[Category: Lippard, S J.]]
[[Category: Lippard, S J.]]
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[[Category: cisplatin]]
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[[Category: Cisplatin]]
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[[Category: deoxyribonucleic acid]]
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[[Category: Deoxyribonucleic acid]]
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[[Category: dna]]
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[[Category: Dna]]
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[[Category: dodecamer]]
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[[Category: Dodecamer]]
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[[Category: duplex]]
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[[Category: Duplex]]
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[[Category: hmg-domain interaction]]
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[[Category: Hmg-domain interaction]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:57:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:35:19 2008''
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Revision as of 06:57, 2 May 2008

Image:1a84.jpg

Template:STRUCTURE 1a84

NMR SOLUTION STRUCTURE OF A DNA DODECAMER DUPLEX CONTAINING A CIS-DIAMMINEPLATINUM(II) D(GPG) INTRASTRAND CROSS-LINK, THE MAJOR ADDUCT OF THE ANTICANCER DRUG CISPLATIN, 1 STRUCTURE


Overview

The structure of the DNA duplex dodecamer, d(CCTCTGGTCTCC. GGAGACCAGAGG), containing the cisplatin d(GpG) 1,2-intrastrand cross-link at the position denoted by asterisks, was determined in solution by high-resolution 2D NMR spectroscopy and restrained molecular dynamics refinement. The cis-[Pt(NH3)2 inverted question markd(GpG-N7(1), N7(2)) inverted question mark] lesion causes the adjacent guanine bases to roll toward one another by 49 degrees, leading to an overall helix bend angle of 78 degrees. These features are more exaggerated than those observed in the X-ray crystal structure determined for the same platinated duplex [Takahara et al. (1995) Nature 377, 649-652]. A common property of the solution and crystal structures is the widening and flattening of the minor groove opposite the platinum adduct, affording geometric parameters resembling those found in A-form DNA. This deformation is especially noteworthy for the solution structure because its sugar puckers are primarily those of B-form DNA. The unwinding of the helix at the site of platination is 25 degrees. The curvature and shape of the platinated duplex are remarkably similar to those observed in DNA duplexes complexed by the HMG-domain proteins SRY and LEF-1. The structure reveals how cisplatin binding alters DNA in such a manner as to facilitate HMG-domain protein recognition.

About this Structure

Full crystallographic information is available from OCA.

Reference

NMR solution structure of a DNA dodecamer duplex containing a cis-diammineplatinum(II) d(GpG) intrastrand cross-link, the major adduct of the anticancer drug cisplatin., Gelasco A, Lippard SJ, Biochemistry. 1998 Jun 30;37(26):9230-9. PMID:9649303 Page seeded by OCA on Fri May 2 09:57:18 2008

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