1ab4

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1ab4.gif|left|200px]]
[[Image:1ab4.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1ab4 |SIZE=350|CAPTION= <scene name='initialview01'>1ab4</scene>, resolution 2.8&Aring;
+
The line below this paragraph, containing "STRUCTURE_1ab4", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND=
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_topoisomerase_(ATP-hydrolyzing) DNA topoisomerase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.3 5.99.1.3] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1ab4| PDB=1ab4 | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ab4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ab4 OCA], [http://www.ebi.ac.uk/pdbsum/1ab4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ab4 RCSB]</span>
+
-
}}
+
'''59KDA FRAGMENT OF GYRASE A FROM E. COLI'''
'''59KDA FRAGMENT OF GYRASE A FROM E. COLI'''
Line 23: Line 20:
==Reference==
==Reference==
Crystal structure of the breakage-reunion domain of DNA gyrase., Morais Cabral JH, Jackson AP, Smith CV, Shikotra N, Maxwell A, Liddington RC, Nature. 1997 Aug 28;388(6645):903-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9278055 9278055]
Crystal structure of the breakage-reunion domain of DNA gyrase., Morais Cabral JH, Jackson AP, Smith CV, Shikotra N, Maxwell A, Liddington RC, Nature. 1997 Aug 28;388(6645):903-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9278055 9278055]
-
[[Category: DNA topoisomerase (ATP-hydrolyzing)]]
 
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
Line 29: Line 25:
[[Category: Liddington, R C.]]
[[Category: Liddington, R C.]]
[[Category: Maxwell, A.]]
[[Category: Maxwell, A.]]
-
[[Category: gyrase]]
+
[[Category: Gyrase]]
-
[[Category: supercoiling dna]]
+
[[Category: Supercoiling dna]]
-
[[Category: topoisomerase ii]]
+
[[Category: Topoisomerase ii]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:03:54 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:36:50 2008''
+

Revision as of 07:03, 2 May 2008

Image:1ab4.gif


PDB ID 1ab4

Drag the structure with the mouse to rotate
1ab4, resolution 2.80Å ()
Activity: DNA topoisomerase (ATP-hydrolyzing), with EC number 5.99.1.3
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



59KDA FRAGMENT OF GYRASE A FROM E. COLI


Overview

DNA gyrase is a type II DNA topoisomerase from bacteria that introduces supercoils into DNA. It catalyses the breakage of a DNA duplex (the G segment), the passage of another segment (the T segment) through the break, and then the reunification of the break. This activity involves the opening and dosing of a series of molecular 'gates' which is coupled to ATP hydrolysis. Here we present the crystal structure of the 'breakage-reunion' domain of the gyrase at 2.8 A resolution. Comparison of the structure of this 59K (relative molecular mass, 59,000) domain with that of a 92K fragment of yeast topoisomerase II reveals a very different quaternary organization, and we propose that the two structures represent two principal conformations that participate in the enzymatic pathway. The gyrase structure reveals a new dimer contact with a grooved concave surface for binding the G segment and a cluster of conserved charged residues surrounding the active-site tyrosines. It also shows how breakage of the G segment can occur and, together with the topoisomerase II structure, suggests a pathway by which the T segment can be released through the second gate of the enzyme. Mutations that confer resistance to the quinolone antibacterial agents cluster at the new dimer interface, indicating how these drugs might interact with the gyrase-DNA complex.

About this Structure

1AB4 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of the breakage-reunion domain of DNA gyrase., Morais Cabral JH, Jackson AP, Smith CV, Shikotra N, Maxwell A, Liddington RC, Nature. 1997 Aug 28;388(6645):903-6. PMID:9278055 Page seeded by OCA on Fri May 2 10:03:54 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ab4"
Personal tools