3x22
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| + | |||
==Crystal structure of Escherichia coli nitroreductase NfsB mutant N71S/F123A/F124W== | ==Crystal structure of Escherichia coli nitroreductase NfsB mutant N71S/F123A/F124W== | ||
<StructureSection load='3x22' size='340' side='right' caption='[[3x22]], [[Resolution|resolution]] 2.00Å' scene=''> | <StructureSection load='3x22' size='340' side='right' caption='[[3x22]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3x22]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X22 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3X22 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3x22]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X22 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3X22 FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ds7|1ds7]], [[3x21|3x21]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ds7|1ds7]], [[3x21|3x21]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nfnB, dprA, nfsB, nfsI, ntr ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6,7-dihydropteridine_reductase 6,7-dihydropteridine reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.34 1.5.1.34] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6,7-dihydropteridine_reductase 6,7-dihydropteridine reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.34 1.5.1.34] </span></td></tr> | ||
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3x22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x22 OCA], [http://pdbe.org/3x22 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3x22 RCSB], [http://www.ebi.ac.uk/pdbsum/3x22 PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3x22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x22 OCA], [http://pdbe.org/3x22 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3x22 RCSB], [http://www.ebi.ac.uk/pdbsum/3x22 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3x22 ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/NFNB_ECOLI NFNB_ECOLI]] Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. Capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species.<ref>PMID:15684426</ref> | [[http://www.uniprot.org/uniprot/NFNB_ECOLI NFNB_ECOLI]] Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. Capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species.<ref>PMID:15684426</ref> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Nitroreductase|Nitroreductase]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
| Line 15: | Line 20: | ||
</StructureSection> | </StructureSection> | ||
[[Category: 6,7-dihydropteridine reductase]] | [[Category: 6,7-dihydropteridine reductase]] | ||
| + | [[Category: Ecoli]] | ||
[[Category: Bai, J]] | [[Category: Bai, J]] | ||
[[Category: Yang, J]] | [[Category: Yang, J]] | ||
Revision as of 16:48, 27 February 2019
Crystal structure of Escherichia coli nitroreductase NfsB mutant N71S/F123A/F124W
| |||||||||||
