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5dj4

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Revision as of 12:34, 6 November 2017

Leucine-bound Sestrin2 from Homo sapiens

Structural highlights

5dj4 is a 5 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	SESN2, SEST2 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SESN2_HUMAN] Involved in the reduction of peroxiredoxins.^[1]

Publication Abstract from PubMed

Eukaryotic cells coordinate growth with the availability of nutrients through mTOR complex 1 (mTORC1), a master growth regulator. Leucine is of particular importance and activates mTORC1 via the Rag GTPases and their regulators GATOR1 and GATOR2. Sestrin2 interacts with GATOR2 and is a leucine sensor. We present the 2.7-A crystal structure of Sestrin2 in complex with leucine. Leucine binds through a single pocket that coordinates its charged functional groups and confers specificity for the hydrophobic side chain. A loop encloses leucine and forms a lid-latch mechanism required for binding. A structure-guided mutation in Sestrin2 that decreases its affinity for leucine leads to a concomitant increase in the leucine concentration required for mTORC1 activation in cells. These results provide a structural mechanism of amino acid sensing by the mTORC1 pathway.

Structural basis for leucine sensing by the Sestrin2-mTORC1 pathway.,Saxton RA, Knockenhauer KE, Wolfson RL, Chantranupong L, Pacold ME, Wang T, Schwartz TU, Sabatini DM Science. 2015 Nov 19. pii: aad2087. PMID:26586190^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Budanov AV, Sablina AA, Feinstein E, Koonin EV, Chumakov PM. Regeneration of peroxiredoxins by p53-regulated sestrins, homologs of bacterial AhpD. Science. 2004 Apr 23;304(5670):596-600. PMID:15105503 doi:http://dx.doi.org/10.1126/science.1095569
↑ Saxton RA, Knockenhauer KE, Wolfson RL, Chantranupong L, Pacold ME, Wang T, Schwartz TU, Sabatini DM. Structural basis for leucine sensing by the Sestrin2-mTORC1 pathway. Science. 2015 Nov 19. pii: aad2087. PMID:26586190 doi:http://dx.doi.org/10.1126/science.aad2087

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5dj4"

@@ Line 1: / Line 1: @@
 ==Leucine-bound Sestrin2 from Homo sapiens==
 <StructureSection load='5dj4' size='340' side='right' caption='[[5dj4]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5dj4]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DJ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DJ4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5dj4]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DJ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DJ4 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LEU:LEUCINE'>LEU</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dj4 OCA], [http://pdbe.org/5dj4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dj4 RCSB], [http://www.ebi.ac.uk/pdbsum/5dj4 PDBsum]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SESN2, SEST2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dj4 OCA], [http://pdbe.org/5dj4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dj4 RCSB], [http://www.ebi.ac.uk/pdbsum/5dj4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dj4 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Human]]
 [[Category: Knockenhauer, K E]]
 [[Category: Saxton, R A]]

5dj4

From Proteopedia

Revision as of 12:34, 6 November 2017

Leucine-bound Sestrin2 from Homo sapiens

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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