1ag6
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1ag6.jpg|left|200px]] | [[Image:1ag6.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1ag6", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | | | + | {{STRUCTURE_1ag6| PDB=1ag6 | SCENE= }} |
| - | | | + | |
| - | + | ||
| - | }} | + | |
'''PLASTOCYANIN FROM SPINACH''' | '''PLASTOCYANIN FROM SPINACH''' | ||
| Line 28: | Line 25: | ||
[[Category: Xue, Y.]] | [[Category: Xue, Y.]] | ||
[[Category: Young, S.]] | [[Category: Young, S.]] | ||
| - | [[Category: | + | [[Category: Blue-copper protein]] |
| - | [[Category: | + | [[Category: Electron transfer]] |
| - | [[Category: | + | [[Category: Electron transport]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:13:29 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:13, 2 May 2008
PLASTOCYANIN FROM SPINACH
Overview
The crystal structure of plastocyanin from spinach has been determined using molecular replacement, with the structure of plastocyanin from poplar as a search model. Successful crystallization was facilitated by site-directed mutagenesis in which residue Gly8 was substituted with Asp. The region around residue 8 was believed to be too mobile for the wild-type protein to form crystals despite extensive screening. The current structure represents the oxidized plastocyanin, copper (II), at low pH (approximately 4.4). In contrast to the similarity in the core region as compared to its poplar counterpart, the structure shows some significant differences in loop regions. The most notable is the large shift of the 59-61 loop where the largest shift is 3.0 A for the C(alpha) atom of Glu59. This results in different patterns of electrostatic potential around the acidic patches for the two proteins.
About this Structure
1AG6 is a Single protein structure of sequence from Spinacia oleracea. Full crystallographic information is available from OCA.
Reference
Crystal structure of spinach plastocyanin at 1.7 A resolution., Xue Y, Okvist M, Hansson O, Young S, Protein Sci. 1998 Oct;7(10):2099-105. PMID:9792096 Page seeded by OCA on Fri May 2 10:13:29 2008
