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1aly
From Proteopedia
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[[Image:1aly.gif|left|200px]] | [[Image:1aly.gif|left|200px]] | ||
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'''CRYSTAL STRUCTURE OF HUMAN CD40 LIGAND''' | '''CRYSTAL STRUCTURE OF HUMAN CD40 LIGAND''' | ||
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[[Category: Karpusas, M.]] | [[Category: Karpusas, M.]] | ||
[[Category: Thomas, D.]] | [[Category: Thomas, D.]] | ||
| - | [[Category: | + | [[Category: Cd40 ligand]] |
| - | [[Category: | + | [[Category: Cd40l]] |
| - | [[Category: | + | [[Category: Cytokine]] |
| - | [[Category: | + | [[Category: Hyper-igm syndrome]] |
| - | [[Category: | + | [[Category: Tnf]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:26:03 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:26, 2 May 2008
CRYSTAL STRUCTURE OF HUMAN CD40 LIGAND
Overview
BACKGROUND: The CD40 ligand (CD40L) is a member of the tumor necrosis factor (TNF) family of proteins and is transiently expressed on the surface of activated T cells. The binding of CD40L to CD40, which is expressed on the surface of B cells, provides a critical and unique pathway of cellular activation resulting in antibody isotype switching, regulation of apoptosis, and B cell proliferation and differentiation. Naturally occurring mutations of CD40L result in the clinical hyper-IgM syndrome, characterized by an inability to produce immunoglobulins of the IgG, IgA and IgE isotypes. RESULTS: We have determined the crystal structure of a soluble extracellular fragment of human CD40L to 2 A resolution and with an R factor of 21.8%. Although the molecule forms a trimer similar to that found for other members of the TNF family, such as TNF alpha and lymphotoxin-alpha, and exhibits a similar overall fold, there are considerable differences in several loops including those predicted to be involved in CD40 binding. CONCLUSIONS: The structure suggests that most of the hyper-IgM syndrome mutations affect the folding and stability of the molecule rather than the CD40-binding site directly. Despite the fact that the hyper-IgM syndrome mutations are dispersed in the primary sequence, a large fraction of them are clustered in space in the vicinity of a surface loop, close to the predicted CD40-binding site.
About this Structure
1ALY is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
2 A crystal structure of an extracellular fragment of human CD40 ligand., Karpusas M, Hsu YM, Wang JH, Thompson J, Lederman S, Chess L, Thomas D, Structure. 1995 Oct 15;3(10):1031-9. PMID:8589998 Page seeded by OCA on Fri May 2 10:26:03 2008
Categories: Homo sapiens | Single protein | Hsu, Y M. | Karpusas, M. | Thomas, D. | Cd40 ligand | Cd40l | Cytokine | Hyper-igm syndrome | Tnf
