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Sandbox UNLPam 7

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== Crystal structure of a novel two domain
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GH78 family a-rhamnosidase from Klebsiella
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oxytoca with rhamnose bound ==
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==Your Heading Here (maybe something like 'Structure')==
==Your Heading Here (maybe something like 'Structure')==
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<StructureSection load='4xhc' size='340' side='right' caption='Caption for this structure' scene=''>
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<StructureSection load='4xhc' size='350' side='right' caption='X-ray crystal structure of the a-L-rhamnosidase from K. oxytoca (KoRha).' scene=''>
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Anything in this section will appear adjacent to the 3D structure and will be scrollable.
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a-L-rhamnosidases (E.C. 3.2.1.40) are found widely distributed
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in nature and have been reported in animals,
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' size='350' frame='true' align='right' caption='Insert caption here' scene='Insert optional scene name here' />==Your Heading Here (maybe something like 'Structure')==
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plants, yeast, fungi and bacteria, where they are responsible
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<StructureSection load='3rec' size='350' side='right' caption='Escherichia coli reca protein-bound DNA (PDB entry [[3rec]])' scene=''>==Your Heading Here (maybe something like 'Structure')==
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for the cleavage of a-L-rhamnose from a wide range of
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<StructureSection load='3rec' size='350' side='right' caption='Escherichia coli reca protein-bound DNA (PDB entry [[3rec]])' scene=''>
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compounds.1 a-L-rhamnose is found in plants and bacteria
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Anything in this section will appear adjacent to the 3D structure and will be scrollable.
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</StructureSection>==Your Heading Here (maybe something like 'Structure')==
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<StructureSection load='3rec' size='350' side='right' caption='Escherichia coli reca protein-bound DNA (PDB entry [[3rec]])' scene=''>==Your Heading Here (maybe something like 'Structure')==
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<StructureSection load='3rec' size='350' side='right' caption='Escherichia coli reca protein-bound DNA (PDB entry [[3rec]])' scene=''>
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Anything in this section will appear adjacent to the 3D structure and will be scrollable.
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</StructureSection></StructureSection></StructureSection></StructureSection>== Crystal structure of a novel two domain GH78 family a-rhamnosidase from Klebsiella
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oxytoca with rhamnose bound ==
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<StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''>
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== Structure ==
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The crystal structure of the GH78 family a-rhamnosidase from Klebsiella oxytoca (KoRha) has been determined at 2.7 A °
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resolution with rhamnose bound in the active site of the catalytic domain. Curiously, the putative catalytic acid, Asp 222, is
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preceded by an unusual non-proline cis-peptide bond which helps to project the carboxyl group into the active centre. This
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KoRha homodimeric structure is significantly smaller than those of the other previously determined GH78 structures.
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Nevertheless, the enzyme displays a-rhamnosidase activity when assayed in vitro, suggesting that the additional structural
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domains found in the related enzymes are dispensible for function.
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== Function ==
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a-L-rhamnose is found in plants and bacteria
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as components of polysaccharides, such as pectins,2 and
as components of polysaccharides, such as pectins,2 and
the O antigen polysaccharides, responsible for determining
the O antigen polysaccharides, responsible for determining
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and for the release of flavonoids from rhamnosylated precursors;
and for the release of flavonoids from rhamnosylated precursors;
in wine production they play a role in the hydrolysis
in wine production they play a role in the hydrolysis
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of glycosylated terpene aroma compounds.5 In the
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of glycosylated terpene aroma compounds.
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context of expanding and diversifying the suite of enzymes
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available to us for carbohydrate biotransformations,6–13 we
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were drawn to consider a-rhamnosidases.
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In the CAZy database,14 rhamnosidases are currently
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classified into GH28 pectin hydrolases, GH78 containing
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exclusively rhamnosidases, and GH106 containing a single enzyme from Sphingomonas
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== Disease ==
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== Relevance ==
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== Structural highlights ==
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This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.
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</StructureSection>
</StructureSection>
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== References ==
 
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<references/>
 

Revision as of 13:13, 9 December 2015

== Crystal structure of a novel two domain GH78 family a-rhamnosidase from Klebsiella oxytoca with rhamnose bound ==

Your Heading Here (maybe something like 'Structure')

X-ray crystal structure of the a-L-rhamnosidase from K. oxytoca (KoRha).

Drag the structure with the mouse to rotate
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