This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1arh

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1arh.gif|left|200px]]
[[Image:1arh.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1arh |SIZE=350|CAPTION= <scene name='initialview01'>1arh</scene>, resolution 2.3&Aring;
+
The line below this paragraph, containing "STRUCTURE_1arh", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=PPD:2-[(3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYLENE)-AMINO]-SUCCINIC+ACID'>PPD</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1arh| PDB=1arh | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1arh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1arh OCA], [http://www.ebi.ac.uk/pdbsum/1arh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1arh RCSB]</span>
+
-
}}
+
'''ASPARTATE AMINOTRANSFERASE, Y225R/R386A MUTANT'''
'''ASPARTATE AMINOTRANSFERASE, Y225R/R386A MUTANT'''
Line 28: Line 25:
[[Category: Jansonius, J N.]]
[[Category: Jansonius, J N.]]
[[Category: Malashkevich, V N.]]
[[Category: Malashkevich, V N.]]
-
[[Category: transferase (aminotransferase)]]
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:37:11 2008''
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:46:09 2008''
+

Revision as of 07:37, 2 May 2008

Image:1arh.gif

Template:STRUCTURE 1arh

ASPARTATE AMINOTRANSFERASE, Y225R/R386A MUTANT


Overview

The electron distribution in the coenzyme-substrate adduct of aspartate aminotransferase was changed by replacing active-site Arg386 with alanine and introducing a new arginine residue nearby. [Y225R, R386A]Aspartate aminotransferase decarboxylates L-aspartate to L-alanine (kcat = 0.04 s-1), while its transaminase activity towards dicarboxylic amino acids is decreased by three orders of magnitude (kcat = 0.19 s-1). Molecular-dynamics simulations based on the crystal structure of the mutant enzyme suggest that a new hydrogen bond to the imine N atom of the pyridoxal-5'-phosphate- aspartate adduct and an altered electrostatic potential around its beta-carboxylate group underlie the 650,000-fold increase in the ratio of beta-decarboxylase/transaminase activity.

About this Structure

1ARH is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Changing the reaction specificity of a pyridoxal-5'-phosphate-dependent enzyme., Graber R, Kasper P, Malashkevich VN, Sandmeier E, Berger P, Gehring H, Jansonius JN, Christen P, Eur J Biochem. 1995 Sep 1;232(2):686-90. PMID:7556224 Page seeded by OCA on Fri May 2 10:37:11 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1arh"
Personal tools