5c22
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Zn-bound HlyD from E. coli== | |
| + | <StructureSection load='5c22' size='340' side='right' caption='[[5c22]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5c22]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C22 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C22 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5c21|5c21]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c22 OCA], [http://pdbe.org/5c22 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c22 RCSB], [http://www.ebi.ac.uk/pdbsum/5c22 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The protein toxin HlyA of Escherichia coli is exported without a periplasmic intermediate by the type I secretion system (T1SS). The T1SS is composed of an inner membrane ABC transporter HlyB, an outer-membrane channel protein TolC, and a membrane fusion protein HlyD. However, the assembly of the T1SS remains to be elucidated. In this study, we determine the crystal structure of a part of the C-terminal periplasmic domain of HlyD. The long alpha-helical domain consisting of three alpha helices and a lipoyl domain was identified in the crystal structure. Based on the HlyD structure, we modeled the hexameric assembly of HlyD with a long alpha-helical barrel, which formed a complex with TolC in an intermeshing cogwheel-to-cogwheel manner, as observed in tripartite RND-type drug efflux pumps. These observations provide a structural blueprint for understanding the type I secretion system in pathogenic Gram-negative bacteria. | ||
| - | + | Crystal Structure of a Soluble Fragment of the Membrane Fusion Protein HlyD in a Type I Secretion System of Gram-Negative Bacteria.,Kim JS, Song S, Lee M, Lee S, Lee K, Ha NC Structure. 2016 Jan 22. pii: S0969-2126(16)00005-8. doi:, 10.1016/j.str.2015.12.012. PMID:26833388<ref>PMID:26833388</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: Ha, N | + | <div class="pdbe-citations 5c22" style="background-color:#fffaf0;"></div> |
| - | [[Category: Kim, J | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Ha, N C]] | ||
| + | [[Category: Kim, J S]] | ||
| + | [[Category: Hlyd]] | ||
| + | [[Category: Multicrystal averaging]] | ||
| + | [[Category: Protein transport]] | ||
| + | [[Category: Type i secretion system]] | ||
| + | [[Category: Zn sad]] | ||
Revision as of 02:45, 21 February 2016
Crystal structure of Zn-bound HlyD from E. coli
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