1aw1

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1aw1.gif|left|200px]]
[[Image:1aw1.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1aw1 |SIZE=350|CAPTION= <scene name='initialview01'>1aw1</scene>, resolution 2.7&Aring;
+
The line below this paragraph, containing "STRUCTURE_1aw1", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=PGA:2-PHOSPHOGLYCOLIC+ACID'>PGA</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1aw1| PDB=1aw1 | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1aw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aw1 OCA], [http://www.ebi.ac.uk/pdbsum/1aw1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1aw1 RCSB]</span>
+
-
}}
+
'''TRIOSEPHOSPHATE ISOMERASE OF VIBRIO MARINUS COMPLEXED WITH 2-PHOSPHOGLYCOLATE'''
'''TRIOSEPHOSPHATE ISOMERASE OF VIBRIO MARINUS COMPLEXED WITH 2-PHOSPHOGLYCOLATE'''
Line 29: Line 26:
[[Category: Wierenga, R K.]]
[[Category: Wierenga, R K.]]
[[Category: Zeelen, J P.]]
[[Category: Zeelen, J P.]]
-
[[Category: isomerase]]
+
[[Category: Isomerase]]
-
[[Category: psychrophilic]]
+
[[Category: Psychrophilic]]
-
[[Category: vibrio marinus]]
+
[[Category: Vibrio marinus]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:45:51 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:48:41 2008''
+

Revision as of 07:45, 2 May 2008

Image:1aw1.gif

Template:STRUCTURE 1aw1

TRIOSEPHOSPHATE ISOMERASE OF VIBRIO MARINUS COMPLEXED WITH 2-PHOSPHOGLYCOLATE


Overview

The purification and characterization of triose-phosphate isomerase from the psychrophilic bacterium Vibrio marinus (vTIM) is described. Crystal structures of the vTIM-sulfate complex and the vTIM-2-phosphoglycolate complex (at a 2.7-A resolution) are also presented. The optimal growth temperature of Vibrio marinus is 15 degrees C. Stability studies show that vTIM is an unstable protein with a half-life of only 10 min at 25 degrees C. The vTIM sequence is most closely related to the sequence of Escherichia coli TIM (eTIM) (66% identity), and several unique structural features described for eTIM are also seen in vTIM, but eTIM is considerably more stable. The Td values of vTIM and eTIM, determined by calorimetric studies, are 41 and 54 degrees C, respectively. Amino acid sequence comparison reveals that vTIM has an alanine in loop 8 (at position 238), whereas all other TIM sequences known to date have a serine. The vTIM mutant A238S was produced and characterized. Compared with wild type, the catalytic efficiency of the A238S mutant is somewhat reduced, and its stability is considerably increased.

About this Structure

1AW1 is a Single protein structure of sequence from Moritella marina. Full crystallographic information is available from OCA.

Reference

Triose-phosphate isomerase (TIM) of the psychrophilic bacterium Vibrio marinus. Kinetic and structural properties., Alvarez M, Zeelen JP, Mainfroid V, Rentier-Delrue F, Martial JA, Wyns L, Wierenga RK, Maes D, J Biol Chem. 1998 Jan 23;273(4):2199-206. PMID:9442062 Page seeded by OCA on Fri May 2 10:45:51 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1aw1"
Personal tools