1ayz

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[[Image:1ayz.jpg|left|200px]]
[[Image:1ayz.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1ayz |SIZE=350|CAPTION= <scene name='initialview01'>1ayz</scene>, resolution 2.60&Aring;
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The line below this paragraph, containing "STRUCTURE_1ayz", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=NUL:Acceptor+Residue+For+Activated+C-Terminus+Of+Ubiquitin'>NUL</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND=
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1ayz| PDB=1ayz | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ayz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ayz OCA], [http://www.ebi.ac.uk/pdbsum/1ayz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ayz RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF THE SACCHAROMYCES CEREVISIAE UBIQUITIN-CONJUGATING ENZYME RAD6 (UBC2) AT 2.6A RESOLUTION'''
'''CRYSTAL STRUCTURE OF THE SACCHAROMYCES CEREVISIAE UBIQUITIN-CONJUGATING ENZYME RAD6 (UBC2) AT 2.6A RESOLUTION'''
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[[Category: Prakash, S.]]
[[Category: Prakash, S.]]
[[Category: Worthylake, D K.]]
[[Category: Worthylake, D K.]]
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[[Category: ubiquitin conjugation]]
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[[Category: Ubiquitin conjugation]]
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[[Category: ubiquitin-conjugating enzyme]]
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[[Category: Ubiquitin-conjugating enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:51:49 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:50:25 2008''
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Revision as of 07:51, 2 May 2008

Image:1ayz.jpg

Template:STRUCTURE 1ayz

CRYSTAL STRUCTURE OF THE SACCHAROMYCES CEREVISIAE UBIQUITIN-CONJUGATING ENZYME RAD6 (UBC2) AT 2.6A RESOLUTION


Overview

The Saccharomyces cerevisiae ubiquitin-conjugating enzyme (UBC) Rad6 is required for several functions, including the repair of UV damaged DNA, damage-induced mutagenesis, sporulation, and the degradation of cellular proteins that possess destabilizing N-terminal residues. Rad6 mediates its role in N-end rule-dependent protein degradation via interaction with the ubiquitin-protein ligase Ubr1 and in DNA repair via interactions with the DNA binding protein Rad18. We report here the crystal structure of Rad6 refined at 2.6 A resolution to an R factor of 21.3%. The protein adopts an alpha/beta fold that is very similar to other UBC structures. An apparent difference at the functionally important first helix, however, has prompted a reassessment of previously reported structures. The active site cysteine lies in a cleft formed by a coil region that includes the 310 helix and a loop that is in different conformations for the three molecules in the asymmetric unit. Residues important for Rad6 interaction with Ubr1 and Rad18 are on the opposite side of the structure from the active site, indicating that this part of the UBC surface participates in protein-protein interactions that define Rad6 substrate specificity.

About this Structure

1AYZ is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Crystal structure of the Saccharomyces cerevisiae ubiquitin-conjugating enzyme Rad6 at 2.6 A resolution., Worthylake DK, Prakash S, Prakash L, Hill CP, J Biol Chem. 1998 Mar 13;273(11):6271-6. PMID:9497353 Page seeded by OCA on Fri May 2 10:51:49 2008

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