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1b0l

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{{Structure
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b0l OCA], [http://www.ebi.ac.uk/pdbsum/1b0l PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1b0l RCSB]</span>
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'''RECOMBINANT HUMAN DIFERRIC LACTOFERRIN'''
'''RECOMBINANT HUMAN DIFERRIC LACTOFERRIN'''
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[[Category: Jameson, G B.]]
[[Category: Jameson, G B.]]
[[Category: Sun, X.]]
[[Category: Sun, X.]]
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[[Category: binding protein]]
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[[Category: Binding protein]]
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[[Category: metalloprotein]]
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[[Category: Metalloprotein]]
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[[Category: transferrin]]
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[[Category: Transferrin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:55:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:51:13 2008''
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Revision as of 07:55, 2 May 2008

Image:1b0l.gif

Template:STRUCTURE 1b0l

RECOMBINANT HUMAN DIFERRIC LACTOFERRIN


Overview

Human lactoferrin (hLf) has considerable potential as a therapeutic agent. Overexpression of hLf in the fungus Aspergillus awamori has resulted in the availability of very large quantities of this protein. Here, the three-dimensional structure of the recombinant hLf has been determined by X-ray crystallography at a resolution of 2.2 A. The final model, comprising 5339 protein atoms (residues 1-691, 294 solvent molecules, two Fe3+and two CO32- ions), gives an R factor of 0.181 (free R = 0.274) after refinement against 32231 reflections in the resolution range 10-2.2 A. Superposition of the recombinant hLf structure onto the native milk hLf structure shows a very high level of correspondence; the main-chain atoms for the entire polypeptide can be superimposed with an r.m.s. deviation of only 0.3 A and there are no significant differences in side-chain conformations or in the iron-binding sites. Dynamic properties, as measured by B-value distributions or iron-release kinetics, also agree closely. This shows that the structure of the protein is not affected by the mode of expression, the use of strain-improvement procedures or the changes in glycosylation due to the fungal system.

About this Structure

1B0L is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of recombinant human lactoferrin expressed in Aspergillus awamori., Sun XL, Baker HM, Shewry SC, Jameson GB, Baker EN, Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):403-7. PMID:10089347 Page seeded by OCA on Fri May 2 10:55:22 2008

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