1b3c
From Proteopedia
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'''SOLUTION STRUCTURE OF A BETA-NEUROTOXIN FROM THE NEW WORLD SCORPION CENTRUROIDES SCULPTURATUS EWING''' | '''SOLUTION STRUCTURE OF A BETA-NEUROTOXIN FROM THE NEW WORLD SCORPION CENTRUROIDES SCULPTURATUS EWING''' | ||
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[[Category: Trent, J O.]] | [[Category: Trent, J O.]] | ||
[[Category: Watt, D D.]] | [[Category: Watt, D D.]] | ||
| - | [[Category: | + | [[Category: New world toxin]] |
| - | [[Category: | + | [[Category: Scorpion neurotoxin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:00:33 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 08:00, 2 May 2008
SOLUTION STRUCTURE OF A BETA-NEUROTOXIN FROM THE NEW WORLD SCORPION CENTRUROIDES SCULPTURATUS EWING
Overview
We report the detailed solution structure of the 7.2 kDa protein CsE-I, a beta-neurotoxin from the New World scorpion Centruroides sculpturatus Ewing. This toxin binds to sodium channels, but unlike the alpha-neurotoxins, shifts the voltage of activation toward more negative potentials causing the membrane to fire spontaneously. Sequence-specific proton NMR assignments were made using 600 MHz 2D-NMR data. Distance geometry and dynamical simulated annealing refinements were performed using experimental distance and torsion angle constraints from NOESY and pH-COSY data. A family of 40 structures without constraint violations was generated, and an energy-minimized average structure was computed. The backbone conformation of the CsE-I toxin shows similar secondary structural features as the prototypical alpha-neurotoxin, CsE-v3, and is characterized by a short 2(1/2)-turn alpha-helix and a 3-strand antiparallel beta-sheet, both held together by disulfide bridges. The RMSD for the backbone atoms between CsE-I and CsE-v3 is 1.48 A. Despite this similarity in the overall backbone folding, the these two proteins show some important differences in the primary structure (sequence) and electrostatic potential surfaces. Our studies provide a basis for unravelling the role of these differences in relation to the known differences in the receptor sites on the voltage sensitive sodium channel for the alpha- and beta-neurotoxins.
About this Structure
1B3C is a Single protein structure of sequence from Centruroides sculpturatus. Full crystallographic information is available from OCA.
Reference
Solution structure of a beta-neurotoxin from the New World scorpion Centruroides sculpturatus Ewing., Jablonsky MJ, Jackson PL, Trent JO, Watt DD, Krishna NR, Biochem Biophys Res Commun. 1999 Jan 19;254(2):406-12. PMID:9918851 Page seeded by OCA on Fri May 2 11:00:33 2008
