1b3p
From Proteopedia
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[[Image:1b3p.gif|left|200px]] | [[Image:1b3p.gif|left|200px]] | ||
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| - | + | {{STRUCTURE_1b3p| PDB=1b3p | SCENE= }} | |
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'''5'-D(*GP*GP*AP*GP*GP*AP*T)-3'''' | '''5'-D(*GP*GP*AP*GP*GP*AP*T)-3'''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3P OCA]. | |
==Reference== | ==Reference== | ||
Interlocked mismatch-aligned arrowhead DNA motifs., Kettani A, Bouaziz S, Skripkin E, Majumdar A, Wang W, Jones RA, Patel DJ, Structure. 1999 Jul 15;7(7):803-15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10425682 10425682] | Interlocked mismatch-aligned arrowhead DNA motifs., Kettani A, Bouaziz S, Skripkin E, Majumdar A, Wang W, Jones RA, Patel DJ, Structure. 1999 Jul 15;7(7):803-15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10425682 10425682] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Bouaziz, S.]] | [[Category: Bouaziz, S.]] | ||
[[Category: Jones, R A.]] | [[Category: Jones, R A.]] | ||
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[[Category: Skripkin, E.]] | [[Category: Skripkin, E.]] | ||
[[Category: Wang, W.]] | [[Category: Wang, W.]] | ||
| - | [[Category: (g-g-a) triplet repeat]] | ||
[[Category: 15n-labeled dna]] | [[Category: 15n-labeled dna]] | ||
| - | [[Category: | + | [[Category: Mismatch alignment]] |
| - | [[Category: | + | [[Category: Parallel-stranded segment]] |
| - | [[Category: | + | [[Category: Uniform 13c]] |
| - | [[Category: | + | [[Category: V-shaped backbone]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:01:29 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 08:01, 2 May 2008
5'-D(*GP*GP*AP*GP*GP*AP*T)-3'
Overview
BACKGROUND: Triplet repeat sequences are of considerable biological importance as the expansion of such tandem arrays can lead to the onset of a range of human diseases. Such sequences can self-pair via mismatch alignments to form higher order structures that have the potential to cause replication blocks, followed by strand slippage and sequence expansion. The all-purine d(GGA)n triplet repeat sequence is of particular interest because purines can align via G.G, A.A and G.A mismatch formation. RESULTS: We have solved the structure of the uniformly 13C,15N-labeled d(G1-G2-A3-G4-G5-A6-T7) sequence in 10 mM Na+ solution. This sequence adopts a novel twofold-symmetric duplex fold where interlocked V-shaped arrowhead motifs are aligned solely via interstrand G1.G4, G2.G5 and A3.A6 mismatch formation. The tip of the arrowhead motif is centered about the p-A3-p step, and symmetry-related local parallel-stranded duplex domains are formed by the G1-G2-A3 and G4-G5-A6 segments of partner strands. CONCLUSIONS: The purine-rich (GGA)n triplet repeat sequence is dispersed throughout the eukaryotic genome. Several features of the arrowhead duplex motif for the (GGA)2 triplet repeat provide a unique scaffold for molecular recognition. These include the large localized bend in the sugar-phosphate backbones, the segmental parallel-stranded alignment of strands and the exposure of the Watson-Crick edges of several mismatched bases.
About this Structure
Full crystallographic information is available from OCA.
Reference
Interlocked mismatch-aligned arrowhead DNA motifs., Kettani A, Bouaziz S, Skripkin E, Majumdar A, Wang W, Jones RA, Patel DJ, Structure. 1999 Jul 15;7(7):803-15. PMID:10425682 Page seeded by OCA on Fri May 2 11:01:29 2008
