1b5t

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[[Image:1b5t.gif|left|200px]]
[[Image:1b5t.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1b5t |SIZE=350|CAPTION= <scene name='initialview01'>1b5t</scene>, resolution 2.50&Aring;
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The line below this paragraph, containing "STRUCTURE_1b5t", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.5 1.7.99.5] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= METF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_1b5t| PDB=1b5t | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b5t OCA], [http://www.ebi.ac.uk/pdbsum/1b5t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1b5t RCSB]</span>
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}}
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'''ESCHERICHIA COLI METHYLENETETRAHYDROFOLATE REDUCTASE'''
'''ESCHERICHIA COLI METHYLENETETRAHYDROFOLATE REDUCTASE'''
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[[Category: Sheppard, C A.]]
[[Category: Sheppard, C A.]]
[[Category: Tran, P.]]
[[Category: Tran, P.]]
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[[Category: beta alpha barrel]]
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[[Category: Beta alpha barrel]]
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[[Category: reductase]]
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[[Category: Reductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:06:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:54:12 2008''
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Revision as of 08:06, 2 May 2008

Image:1b5t.gif

Template:STRUCTURE 1b5t

ESCHERICHIA COLI METHYLENETETRAHYDROFOLATE REDUCTASE


Overview

Elevated plasma homocysteine levels are associated with increased risk for cardiovascular disease and neural tube defects in humans. Folate treatment decreases homocysteine levels and dramatically reduces the incidence of neural tube defects. The flavoprotein methylenetetrahydrofolate reductase (MTHFR) is a likely target for these actions of folate. The most common genetic cause of mildly elevated plasma homocysteine in humans is the MTHFR polymorphism A222V (base change C677-->T). The X-ray analysis of E. coli MTHFR, reported here, provides a model for the catalytic domain that is shared by all MTHFRs. This domain is a beta8alpha8 barrel that binds FAD in a novel fashion. Ala 177, corresponding to Ala 222 in human MTHFR, is near the bottom of the barrel and distant from the FAD. The mutation A177V does not affect Km or k(cat) but instead increases the propensity for bacterial MTHFR to lose its essential flavin cofactor. Folate derivatives protect wild-type and mutant E. coli enzymes against flavin loss, and protect human MTHFR and the A222V mutant against thermal inactivation, suggesting a mechanism by which folate treatment reduces homocysteine levels.

About this Structure

1B5T is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The structure and properties of methylenetetrahydrofolate reductase from Escherichia coli suggest how folate ameliorates human hyperhomocysteinemia., Guenther BD, Sheppard CA, Tran P, Rozen R, Matthews RG, Ludwig ML, Nat Struct Biol. 1999 Apr;6(4):359-65. PMID:10201405 Page seeded by OCA on Fri May 2 11:06:24 2008

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