1b8j

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[[Image:1b8j.gif|left|200px]]
[[Image:1b8j.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1b8j |SIZE=350|CAPTION= <scene name='initialview01'>1b8j</scene>, resolution 1.90&Aring;
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The line below this paragraph, containing "STRUCTURE_1b8j", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SVA:SERINE+VANADATE'>SVA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= PHOA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_1b8j| PDB=1b8j | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b8j OCA], [http://www.ebi.ac.uk/pdbsum/1b8j PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1b8j RCSB]</span>
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}}
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'''ALKALINE PHOSPHATASE COMPLEXED WITH VANADATE'''
'''ALKALINE PHOSPHATASE COMPLEXED WITH VANADATE'''
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[[Category: Kantrowitz, E R.]]
[[Category: Kantrowitz, E R.]]
[[Category: Stec, B.]]
[[Category: Stec, B.]]
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[[Category: alcohol acceptor)]]
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[[Category: Alkaline phosphatase]]
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[[Category: alkaline phosphatase]]
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[[Category: Hydrolase]]
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[[Category: hydrolase]]
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[[Category: Phosphoric monoester]]
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[[Category: phosphoric monoester]]
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[[Category: Vanadate]]
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[[Category: transferase (phospho]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:12:15 2008''
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[[Category: vanadate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:55:46 2008''
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Revision as of 08:12, 2 May 2008

Image:1b8j.gif

Template:STRUCTURE 1b8j

ALKALINE PHOSPHATASE COMPLEXED WITH VANADATE


Overview

A high resolution crystal structure of Escherichia coli alkaline phosphatase in the presence of vanadate has been refined to 1.9 A resolution. The vanadate ion takes on a trigonal bipyramidal geometry and is covalently bound by the active site serine nucleophile. A coordinated water molecule occupies the axial position opposite the serine nucleophile, whereas the equatorial oxygen atoms of the vanadate ion are stabilized by interactions with both Arg-166 and the zinc metal ions of the active site. This structural complex supports the in-line displacement mechanism of phosphomonoester hydrolysis by alkaline phosphatase and provides a model for the proposed transition state in the enzyme-catalyzed reaction.

About this Structure

1B8J is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

A model of the transition state in the alkaline phosphatase reaction., Holtz KM, Stec B, Kantrowitz ER, J Biol Chem. 1999 Mar 26;274(13):8351-4. PMID:10085061 Page seeded by OCA on Fri May 2 11:12:15 2008

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