This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1b8j

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1b8j.gif|left|200px]]
[[Image:1b8j.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1b8j |SIZE=350|CAPTION= <scene name='initialview01'>1b8j</scene>, resolution 1.90&Aring;
+
The line below this paragraph, containing "STRUCTURE_1b8j", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SVA:SERINE+VANADATE'>SVA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE= PHOA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1b8j| PDB=1b8j | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b8j OCA], [http://www.ebi.ac.uk/pdbsum/1b8j PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1b8j RCSB]</span>
+
-
}}
+
'''ALKALINE PHOSPHATASE COMPLEXED WITH VANADATE'''
'''ALKALINE PHOSPHATASE COMPLEXED WITH VANADATE'''
Line 29: Line 26:
[[Category: Kantrowitz, E R.]]
[[Category: Kantrowitz, E R.]]
[[Category: Stec, B.]]
[[Category: Stec, B.]]
-
[[Category: alcohol acceptor)]]
+
[[Category: Alkaline phosphatase]]
-
[[Category: alkaline phosphatase]]
+
[[Category: Hydrolase]]
-
[[Category: hydrolase]]
+
[[Category: Phosphoric monoester]]
-
[[Category: phosphoric monoester]]
+
[[Category: Vanadate]]
-
[[Category: transferase (phospho]]
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:12:15 2008''
-
[[Category: vanadate]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:55:46 2008''
+

Revision as of 08:12, 2 May 2008

Image:1b8j.gif

Template:STRUCTURE 1b8j

ALKALINE PHOSPHATASE COMPLEXED WITH VANADATE


Overview

A high resolution crystal structure of Escherichia coli alkaline phosphatase in the presence of vanadate has been refined to 1.9 A resolution. The vanadate ion takes on a trigonal bipyramidal geometry and is covalently bound by the active site serine nucleophile. A coordinated water molecule occupies the axial position opposite the serine nucleophile, whereas the equatorial oxygen atoms of the vanadate ion are stabilized by interactions with both Arg-166 and the zinc metal ions of the active site. This structural complex supports the in-line displacement mechanism of phosphomonoester hydrolysis by alkaline phosphatase and provides a model for the proposed transition state in the enzyme-catalyzed reaction.

About this Structure

1B8J is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

A model of the transition state in the alkaline phosphatase reaction., Holtz KM, Stec B, Kantrowitz ER, J Biol Chem. 1999 Mar 26;274(13):8351-4. PMID:10085061 Page seeded by OCA on Fri May 2 11:12:15 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1b8j"
Personal tools