1bhn

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[[Image:1bhn.gif|left|200px]]
[[Image:1bhn.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1bhn |SIZE=350|CAPTION= <scene name='initialview01'>1bhn</scene>, resolution 2.4&Aring;
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The line below this paragraph, containing "STRUCTURE_1bhn", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=A:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>A</scene>, <scene name='pdbsite=AND F:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>AND F</scene>, <scene name='pdbsite=B:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>B</scene>, <scene name='pdbsite=C:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>C</scene>, <scene name='pdbsite=D:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>D</scene> and <scene name='pdbsite=E:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>E</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=35G:GUANOSINE-3&#39;,5&#39;-MONOPHOSPHATE'>35G</scene>, <scene name='pdbligand=GDP:GUANOSINE-5&#39;-DIPHOSPHATE'>GDP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1bhn| PDB=1bhn | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bhn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bhn OCA], [http://www.ebi.ac.uk/pdbsum/1bhn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bhn RCSB]</span>
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}}
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'''NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM A FROM BOVINE RETINA'''
'''NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM A FROM BOVINE RETINA'''
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[[Category: Ridge, K D.]]
[[Category: Ridge, K D.]]
[[Category: Tordova, M.]]
[[Category: Tordova, M.]]
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[[Category: phosphotransferase]]
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[[Category: Phosphotransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:31:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:01:22 2008''
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Revision as of 08:31, 2 May 2008

Image:1bhn.gif

Template:STRUCTURE 1bhn

NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM A FROM BOVINE RETINA


Overview

The crystal structures of two isoforms of nucleoside diphosphate kinase from bovine retina overexpressed in Escherischia coli have been determined to 2.4 A resolution. Both the isoforms, NBR-A and NBR-B, are hexameric and the fold of the monomer is in agreement with NDP-kinase structures from other biological sources. Although the polypeptide chains of the two isoforms differ by only two residues, they crystallize in different space groups. NBR-A crystallizes in space group P212121 with an entire hexamer in the asymmetric unit, while NBR-B crystallizes in space group P43212 with a trimer in the asymmetric unit. The highly conserved nucleotide-binding site observed in other nucleoside diphosphate kinase structures is also observed here. Both NBR-A and NBR-B were crystallized in the presence of cGMP. The nucleotide is bound with the base in the anti conformation. The NBR-A active site contained both cGMP and GDP each bound at half occupancy. Presumably, NBR-A had retained GDP (or GTP) from the purification process. The NBR-B active site contained only cGMP.

About this Structure

1BHN is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

The three-dimensional structures of two isoforms of nucleoside diphosphate kinase from bovine retina., Ladner JE, Abdulaev NG, Kakuev DL, Tordova M, Ridge KD, Gilliland GL, Acta Crystallogr D Biol Crystallogr. 1999 Jun;55(Pt 6):1127-35. PMID:10329774 Page seeded by OCA on Fri May 2 11:31:29 2008

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