1bjr

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[[Image:1bjr.gif|left|200px]]
[[Image:1bjr.gif|left|200px]]
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{{Structure
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|PDB= 1bjr |SIZE=350|CAPTION= <scene name='initialview01'>1bjr</scene>, resolution 2.44&Aring;
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The line below this paragraph, containing "STRUCTURE_1bjr", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptidase_K Peptidase K], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.64 3.4.21.64] </span>
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{{STRUCTURE_1bjr| PDB=1bjr | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bjr OCA], [http://www.ebi.ac.uk/pdbsum/1bjr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bjr RCSB]</span>
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'''COMPLEX FORMED BETWEEN PROTEOLYTICALLY GENERATED LACTOFERRIN FRAGMENT AND PROTEINASE K'''
'''COMPLEX FORMED BETWEEN PROTEOLYTICALLY GENERATED LACTOFERRIN FRAGMENT AND PROTEINASE K'''
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[[Category: Sharma, S.]]
[[Category: Sharma, S.]]
[[Category: Singh, T P.]]
[[Category: Singh, T P.]]
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[[Category: complex (hydrolase/iron transport)]]
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[[Category: Hydrolase]]
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[[Category: hydrolase]]
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[[Category: Inhibitor]]
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[[Category: inhibitor]]
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[[Category: Lactoferrin]]
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[[Category: lactoferrin]]
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[[Category: Proteinase k]]
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[[Category: proteinase k]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:36:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:02:28 2008''
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Revision as of 08:36, 2 May 2008

Template:STRUCTURE 1bjr

COMPLEX FORMED BETWEEN PROTEOLYTICALLY GENERATED LACTOFERRIN FRAGMENT AND PROTEINASE K


Overview

Lactoferrin is an iron binding glycoprotein with a molecular weight of 80 kDa. The molecule is divided into two lobes representing the N-terminal and C-terminal halves of the polypeptide chain, each containing an iron binding site. The serine proteinases such as trypsin, chymotrypsin, and pepsin hydrolyze lactoferrin into two unequal halves while proteinase K divides this protein into two equal halves. In the first step of hydrolysis by proteinase K, the C- and N-lobes, each having a molecular weight of approximately 40 kDa, are generated. In the next step, the lobes are further hydrolyzed into small molecular weight peptides. The proteinase K isolated from the hydrolyzed product does not show enzymatic activity suggesting that the enzyme is inhibited. Furthermore, the hydrolysis experiments on N-lobe and C-lobe showed that the inhibitory fragment came from the C-lobe. The purified lactoferrin fragment was found to be a decapeptide with an amino acid sequence of H2N-Val-Ala-Gln-Gly-Ala-Ala-Gly-Leu-Ala-COOH. The complex formed between proteinase K and lactoferrin fragment was crystallized by microdialysis. The crystals belonged to the monoclinic space group P2(1) with cell dimensions a = 44.4 A, b = 38.6 A, c = 79.2 A, beta = 105.8 degrees and Z = 2. The crystal structure has been determined at 2.4 A resolution. It has been refined to an R factor of 0.163 for 9044 reflections. The Lf-fragment forms several intermolecular interactions with proteinase K. The Ser-224 Ogamma and His-57 N epsilon2 move away to a distance of 3.68 A in the complex. In the crystal structure, Gln-3I (I indicates inhibitor i.e., lactoferrin fragment) is involved in a direct intermolecular interaction with a symmetry related proteinase K molecule through a strong hydrogen bond with Asp-254. The mode of intermolecular interactions in the complex conformational features of the enzyme and placement of the fragment with respect to the enzyme resemble with the molecular complex of proteinase K with its natural inhibitor PKI3 from wheat.

About this Structure

1BJR is a Single protein structure of sequence from Bubalus bubalis and Engyodontium album. Full crystallographic information is available from OCA.

Reference

Crystal structure of a complex formed between proteolytically-generated lactoferrin fragment and proteinase K., Singh TP, Sharma S, Karthikeyan S, Betzel C, Bhatia KL, Proteins. 1998 Oct 1;33(1):30-8. PMID:9741842 Page seeded by OCA on Fri May 2 11:36:24 2008

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