5cm8

From Proteopedia

(Difference between revisions)

Revision as of 11:45, 27 March 2020

Structural Basis for the Selectivity of Guanine Nucleotide Exchange Factors for the small G-protein Ral

Structural highlights

5cm8 is a 2 chain structure with sequence from Drome and Lk3 transgenic mice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	5cm9
Gene:	Rgl2, Rab2l, Rlf (LK3 transgenic mice), Rala, CG2849 (DROME)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RGL2_MOUSE] Probable guanine nucleotide exchange factor. Putative effector of Ras and/or Rap. Associates with the GTP-bound form of Rap 1A and H-Ras in vitro.

Publication Abstract from PubMed

CDC25 homology domain (CDC25-HD) containing Guanine Nucleotide Exchange Factors (GEFs) initiate signalling by small G-proteins of the Ras-family. Each GEF acts on a small subset of the G-proteins only, thus providing signalling selectivity. Rlf is a GEF with selectivity for the G-proteins RalA and RalB. Here the crystal structure of Rlf in complex with Ral is determined. The Rlf.Ral complex crystallised into two different crystal forms, which represent different steps of the exchange reaction. Thereby general insight in the CDC25-HD catalysed nucleotide exchange is obtained. In addition, the basis for the selectivity of the interaction is investigated. The exchange activity is monitored by the use of recombinant proteins. Selectivity determinants in the binding interface are identified and confirmed by a mutational study.

The structure of the Guanine Nucleotide Exchange Factor Rlf in complex with the small G-protein Ral identifies conformational intermediates of the exchange reaction and the basis for the selectivity.,Popovic M, Schouten A, Rensen-de Leeuw M, Rehmann H J Struct Biol. 2015 Dec 11. pii: S1047-8477(15)30112-X. doi:, 10.1016/j.jsb.2015.12.006. PMID:26687416^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Popovic M, Schouten A, Rensen-de Leeuw M, Rehmann H. The structure of the Guanine Nucleotide Exchange Factor Rlf in complex with the small G-protein Ral identifies conformational intermediates of the exchange reaction and the basis for the selectivity. J Struct Biol. 2015 Dec 11. pii: S1047-8477(15)30112-X. doi:, 10.1016/j.jsb.2015.12.006. PMID:26687416 doi:http://dx.doi.org/10.1016/j.jsb.2015.12.006

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5cm8"

@@ Line 1: / Line 1: @@
 ==Structural Basis for the Selectivity of Guanine Nucleotide Exchange Factors for the small G-protein Ral==
-<StructureSection load='5cm8' size='340' side='right' caption='[[5cm8]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
+<StructureSection load='5cm8' size='340' side='right'caption='[[5cm8]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5cm8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CM8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5cm8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CM8 FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5cm9|5cm9]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cm8 OCA], [http://pdbe.org/5cm8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cm8 RCSB], [http://www.ebi.ac.uk/pdbsum/5cm8 PDBsum]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Rgl2, Rab2l, Rlf ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), Rala, CG2849 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cm8 OCA], [http://pdbe.org/5cm8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cm8 RCSB], [http://www.ebi.ac.uk/pdbsum/5cm8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cm8 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Drome]]
+[[Category: Large Structures]]
+[[Category: Lk3 transgenic mice]]
 [[Category: Popovic, M]]
 [[Category: Rehmann, H]]

5cm8

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Revision as of 11:45, 27 March 2020

Structural Basis for the Selectivity of Guanine Nucleotide Exchange Factors for the small G-protein Ral

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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