4v3r
From Proteopedia
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==Designed armadillo repeat protein with 5 internal repeats, 2nd generation C-cap and 3rd generation N-cap.== | ==Designed armadillo repeat protein with 5 internal repeats, 2nd generation C-cap and 3rd generation N-cap.== | ||
<StructureSection load='4v3r' size='340' side='right' caption='[[4v3r]], [[Resolution|resolution]] 1.95Å' scene=''> | <StructureSection load='4v3r' size='340' side='right' caption='[[4v3r]], [[Resolution|resolution]] 1.95Å' scene=''> | ||
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4v3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v3r OCA], [http://pdbe.org/4v3r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4v3r RCSB], [http://www.ebi.ac.uk/pdbsum/4v3r PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4v3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v3r OCA], [http://pdbe.org/4v3r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4v3r RCSB], [http://www.ebi.ac.uk/pdbsum/4v3r PDBsum]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The armadillo repeat serves as a scaffold for the development of modular peptide-recognition modules. In order to develop such a system, three crystal structures of designed armadillo-repeat proteins with third-generation N-caps (YIII-type), four or five internal repeats (M-type) and second-generation C-caps (AII-type) were determined at 1.8 A (His-YIIIM4AII), 2.0 A (His-YIIIM5AII) and 1.95 A (YIIIM5AII) resolution and compared with those of variants with third-generation C-caps. All constructs are full consensus designs in which the internal repeats have exactly the same sequence, and hence identical conformations of the internal repeats are expected. The N-cap and internal repeats M1 to M3 are indeed extremely similar, but the comparison reveals structural differences in internal repeats M4 and M5 and the C-cap. These differences are caused by long-range effects of the C-cap, contacting molecules in the crystal, and the intrinsic design of the repeat. Unfortunately, the rigid-body movement of the C-terminal part impairs the regular arrangement of internal repeats that forms the putative peptide-binding site. The second-generation C-cap improves the packing of buried residues and thereby the stability of the protein. These considerations are useful for future improvements of an armadillo-repeat-based peptide-recognition system. | ||
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| + | Structures of designed armadillo-repeat proteins show propagation of inter-repeat interface effects.,Reichen C, Madhurantakam C, Hansen S, Grutter MG, Pluckthun A, Mittl PR Acta Crystallogr D Struct Biol. 2016 Jan;72(Pt 1):168-75. doi:, 10.1107/S2059798315023116. Epub 2016 Jan 1. PMID:26894544<ref>PMID:26894544</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 4v3r" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 06:54, 2 March 2016
Designed armadillo repeat protein with 5 internal repeats, 2nd generation C-cap and 3rd generation N-cap.
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