4xyh

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==Wild-type full length Mis16 in Schizosaccharomyces japonicus==
==Wild-type full length Mis16 in Schizosaccharomyces japonicus==
<StructureSection load='4xyh' size='340' side='right' caption='[[4xyh]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
<StructureSection load='4xyh' size='340' side='right' caption='[[4xyh]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xyh OCA], [http://pdbe.org/4xyh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xyh RCSB], [http://www.ebi.ac.uk/pdbsum/4xyh PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xyh OCA], [http://pdbe.org/4xyh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xyh RCSB], [http://www.ebi.ac.uk/pdbsum/4xyh PDBsum]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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CENP-A is a centromere-specific histone H3 variant that is required for kinetochore assembly and accurate chromosome segregation. For it to function properly, CENP-A must be specifically localized to centromeres. In fission yeast, Scm3sp and the Mis18 complex, composed of Mis16, Eic1, and Mis18, function as a CENP-A(Cnp1)-specific chaperone and a recruiting factor, respectively, and together ensure accurate delivery of CENP-A(Cnp1) to centromeres. Although how Scm3sp specifically recognizes CENP-A(Cnp1) has been revealed recently, the recruiting mechanism of CENP-A(Cnp1) via the Mis18 complex remains unknown. In this study, we have determined crystal structures of Schizosaccharomyces japonicus Mis16 alone and in complex with the helix 1 of histone H4 (H4alpha1). Crystal structures followed by mutant analysis and affinity pull-downs have revealed that Mis16 recognizes both H4alpha1 and Scm3sp independently within the CENP-A(Cnp1)/H4:Scm3sp complex. This observation suggests that Mis16 gains CENP-A(Cnp1) specificity by recognizing both Scm3sp and histone H4. Our studies provide insights into the molecular mechanisms underlying specific recruitment of CENP-A(Cnp1)/H4:Scm3sp into centromeres.
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Mis16 Independently Recognizes Histone H4 and the CENP-ACnp1-Specific Chaperone Scm3sp.,An S, Kim H, Cho US J Mol Biol. 2015 Oct 9;427(20):3230-40. doi: 10.1016/j.jmb.2015.08.022. Epub 2015, Sep 4. PMID:26343758<ref>PMID:26343758</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4xyh" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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</StructureSection>
</StructureSection>

Revision as of 19:11, 20 February 2016

Wild-type full length Mis16 in Schizosaccharomyces japonicus

4xyh, resolution 2.30Å

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