This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


EPSP synthase

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 10: Line 10:
== Structural insights ==
== Structural insights ==
-
The enzyme has <scene name='57/570585/Two_domains/2'>two domains</scene>, with the active site found in the interdomain cleft. There is a substantial structural change upon substrate binding, resulting in a <scene name='57/570585/Closed_formation/2'>closed formation</scene>. <scene name='57/570585/Cv/3'>See animation of this process</scene>. '''Glyphosate''' (also known as '''Roundup''') occupies the <scene name='57/570585/Cv/8'>binding site</scene> of the second substrate, phosphoenol pyruvate <ref>PMID:11171958</ref>.
+
The enzyme has two domains, with the active site found in the interdomain cleft <scene name='57/570585/Two_domains/2'>(open conformation)</scene>. There is a substantial structural change upon substrate binding, resulting in a <scene name='57/570585/Closed_formation/2'>closed</scene> conformation. <scene name='57/570585/Cv/3'>See animation of this process</scene>. '''Glyphosate''' (also known as '''Roundup''') occupies the <scene name='57/570585/Cv/8'>binding site</scene> of the second substrate, phosphoenol pyruvate <ref>PMID:11171958</ref>.
</StructureSection>
</StructureSection>

Revision as of 15:54, 20 January 2016

Structure of E. coli EPSP synthase complex with shikimate-3-phosphate, the herbicide glyphosate and formic acid (PDB entry 1g6s)

Drag the structure with the mouse to rotate

3D structures of EPSP synthase

Updated on 20-January-2016

References

  1. ↑ Priestman MA, Healy ML, Funke T, Becker A, Schonbrunn E. Molecular basis for the glyphosate-insensitivity of the reaction of 5-enolpyruvylshikimate 3-phosphate synthase with shikimate. FEBS Lett. 2005 Oct 24;579(25):5773-80. PMID:16225867 doi:10.1016/j.febslet.2005.09.066
  2. ↑ Schonbrunn E, Eschenburg S, Shuttleworth WA, Schloss JV, Amrhein N, Evans JN, Kabsch W. Interaction of the herbicide glyphosate with its target enzyme 5-enolpyruvylshikimate 3-phosphate synthase in atomic detail. Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1376-80. PMID:11171958 doi:http://dx.doi.org/10.1073/pnas.98.4.1376

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Ann Taylor, Joel L. Sussman

Retrieved from "http://52.214.119.220/wiki/index.php/EPSP_synthase"
Personal tools