4d74
From Proteopedia
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==1.57 A crystal structure of erwinia amylovora tyrosine phosphatase amsI== | ==1.57 A crystal structure of erwinia amylovora tyrosine phosphatase amsI== | ||
<StructureSection load='4d74' size='340' side='right' caption='[[4d74]], [[Resolution|resolution]] 1.57Å' scene=''> | <StructureSection load='4d74' size='340' side='right' caption='[[4d74]], [[Resolution|resolution]] 1.57Å' scene=''> | ||
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr> | ||
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d74 OCA], [http://pdbe.org/4d74 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4d74 RCSB], [http://www.ebi.ac.uk/pdbsum/4d74 PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d74 OCA], [http://pdbe.org/4d74 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4d74 RCSB], [http://www.ebi.ac.uk/pdbsum/4d74 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4d74 ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
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<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| - | + | AmsI is a low-molecular-weight protein tyrosine phosphatase that regulates the production of amylovoran in the Gram-negative bacterium Erwinia amylovora, a specific pathogen of rosaceous plants such as apple, pear and quince. Amylovoran is an exopolysaccharide that is necessary for successful infection. In order to shed light on AmsI, its structure was solved at 1.57 A resolution at the same pH as its highest measured activity (pH 5.5). In the active site, a water molecule, bridging between the catalytic Arg15 and the reaction-product analogue sulfate, might be representative of the water molecule attacking the phospho-cysteine intermediate in the second step of the reaction mechanism. | |
| - | + | Characterization and 1.57 A resolution structure of the key fire blight phosphatase AmsI from Erwinia amylovora.,Salomone-Stagni M, Musiani F, Benini S Acta Crystallogr F Struct Biol Commun. 2016 Dec 1;72(Pt 12):903-910. Epub 2016, Nov 30. PMID:27917839<ref>PMID:27917839</ref> | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
Revision as of 11:34, 15 December 2016
1.57 A crystal structure of erwinia amylovora tyrosine phosphatase amsI
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