1bw6
From Proteopedia
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'''HUMAN CENTROMERE PROTEIN B (CENP-B) DNA BINDIGN DOMAIN RP1''' | '''HUMAN CENTROMERE PROTEIN B (CENP-B) DNA BINDIGN DOMAIN RP1''' | ||
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]] | [[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]] | ||
[[Category: Yokoyama, S.]] | [[Category: Yokoyama, S.]] | ||
| - | [[Category: | + | [[Category: Centromere protein]] |
| - | [[Category: | + | [[Category: Dna-binding]] |
| - | [[Category: | + | [[Category: Helix-turn-helix]] |
| - | [[Category: | + | [[Category: Riken structural genomics/proteomics initiative]] |
| - | [[Category: | + | [[Category: Rsgi]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:01:37 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 09:01, 2 May 2008
HUMAN CENTROMERE PROTEIN B (CENP-B) DNA BINDIGN DOMAIN RP1
Overview
CENP-B has been suggested to organize arrays of centromere satellite DNA into a higher order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes. The N-terminal portion of CENP-B is a 15 kDa DNA binding domain (DBD) consisting of two repeating units, RP1 and RP2. The DBD specifically binds to the CENP-B box sequence (17 bp) in centromere DNA. We determined the solution structure of human CENP-B DBD RP1 by multi-dimensional 1H, 13C and 15N NMR methods. The CENP-B DBD RP1 structure consists of four helices and has a helix-turn-helix structure. The overall folding is similar to those of some other eukaryotic DBDs, although significant sequence homology with these proteins was not found. The DBD of yeast RAP1, a telomere binding protein, is most similar to CENP-B DBD RP1. We studied the interaction between CENP-B DBD RP1 and the CENP-B box by the use of NMR chemical shift perturbation. The results suggest that CENP-B DBD RP1 interacts with one of the essential regions of the CENP-B box DNA, mainly at the N-terminal basic region, the N-terminal portion of helix 2 and helix 3.
About this Structure
1BW6 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
A helix-turn-helix structure unit in human centromere protein B (CENP-B)., Iwahara J, Kigawa T, Kitagawa K, Masumoto H, Okazaki T, Yokoyama S, EMBO J. 1998 Feb 2;17(3):827-37. PMID:9451007 Page seeded by OCA on Fri May 2 12:01:37 2008
Categories: Homo sapiens | Single protein | Iwahara, J. | Kigawa, T. | Kitagawa, K. | Masumoto, H. | Okazaki, T. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Yokoyama, S. | Centromere protein | Dna-binding | Helix-turn-helix | Riken structural genomics/proteomics initiative | Rsgi | Structural genomic
