This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5ftp
From Proteopedia
(Difference between revisions)
m (Protected "5ftp" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==sulfur SAD phasing of Cdc23Nterm: data collection with a tailored X- ray beam size at 2.69 A wavelength (4.6 keV)== | |
| + | <StructureSection load='5ftp' size='340' side='right' caption='[[5ftp]], [[Resolution|resolution]] 3.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5ftp]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FTP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FTP FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ftp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ftp OCA], [http://pdbe.org/5ftp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ftp RCSB], [http://www.ebi.ac.uk/pdbsum/5ftp PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/APC8_SCHPO APC8_SCHPO]] Component of the anaphase-promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. Has a role in promoting metaphase to anaphase transition via the ubiquitination of specific mitotic substrates.<ref>PMID:10526233</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The capability to reach wavelengths of up to 3.1 A at the newly established EMBL P13 beamline at PETRA III, the new third-generation synchrotron at DESY in Hamburg, provides the opportunity to explore very long wavelengths to harness the sulfur anomalous signal for phase determination. Data collection at lambda = 2.69 A (4.6 keV) allowed the crystal structure determination by sulfur SAD phasing of Cdc23(Nterm), a subunit of the multimeric anaphase-promoting complex (APC/C). At this energy, Cdc23(Nterm) has an expected Bijvoet ratio <|Fanom|>/<F> of 2.2%, with 282 residues, including six cysteines and five methionine residues, and two molecules in the asymmetric unit (65.4 kDa; 12 Cys and ten Met residues). Selectively illuminating two separate portions of the same crystal with an X-ray beam of 50 microm in diameter allowed crystal twinning to be overcome. The crystals diffracted to 3.1 A resolution, with unit-cell parameters a = b = 61.2, c = 151.5 A, and belonged to space group P43. The refined structure to 3.1 A resolution has an R factor of 18.7% and an Rfree of 25.9%. This paper reports the structure solution, related methods and a discussion of the instrumentation. | ||
| - | + | Data collection with a tailored X-ray beam size at 2.69 A wavelength (4.6 keV): sulfur SAD phasing of Cdc23(Nterm).,Cianci M, Groves MR, Barford D, Schneider TR Acta Crystallogr D Struct Biol. 2016 Mar 1;72(Pt 3):403-12. doi:, 10.1107/S2059798315010268. Epub 2016 Mar 1. PMID:26960127<ref>PMID:26960127</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5ftp" style="background-color:#fffaf0;"></div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Barford, D]] | [[Category: Barford, D]] | ||
[[Category: Cianci, M]] | [[Category: Cianci, M]] | ||
| - | [[Category: Schneider, T | + | [[Category: Groves, M R]] |
| + | [[Category: Schneider, T R]] | ||
| + | [[Category: Cell cycle]] | ||
| + | [[Category: Long wavelength]] | ||
| + | [[Category: Low resolution]] | ||
| + | [[Category: Sad phasing]] | ||
| + | [[Category: Soft x-ray]] | ||
| + | [[Category: Sulfur]] | ||
Revision as of 17:32, 10 May 2016
sulfur SAD phasing of Cdc23Nterm: data collection with a tailored X- ray beam size at 2.69 A wavelength (4.6 keV)
| |||||||||||
