1c4l
From Proteopedia
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'''SOLUTION STRUCTURE OF AN RNA DUPLEX INCLUDING A C-U BASE-PAIR''' | '''SOLUTION STRUCTURE OF AN RNA DUPLEX INCLUDING A C-U BASE-PAIR''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C4L OCA]. | |
==Reference== | ==Reference== | ||
Solution structure of an RNA duplex including a C-U base pair., Tanaka Y, Kojima C, Yamazaki T, Kodama TS, Yasuno K, Miyashita S, Ono A, Ono A, Kainosho M, Kyogoku Y, Biochemistry. 2000 Jun 20;39(24):7074-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10852704 10852704] | Solution structure of an RNA duplex including a C-U base pair., Tanaka Y, Kojima C, Yamazaki T, Kodama TS, Yasuno K, Miyashita S, Ono A, Ono A, Kainosho M, Kyogoku Y, Biochemistry. 2000 Jun 20;39(24):7074-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10852704 10852704] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Kainosho, M.]] | [[Category: Kainosho, M.]] | ||
[[Category: Kodama, T S.]] | [[Category: Kodama, T S.]] | ||
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[[Category: Yamazaki, T.]] | [[Category: Yamazaki, T.]] | ||
[[Category: Yasuno, K.]] | [[Category: Yasuno, K.]] | ||
| - | [[Category: | + | [[Category: C-u base pair]] |
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Revision as of 09:19, 2 May 2008
SOLUTION STRUCTURE OF AN RNA DUPLEX INCLUDING A C-U BASE-PAIR
Overview
The formation of the C-U base pair in a duplex was observed in solution by means of the temperature profile of (15)N chemical shifts, and the precise geometry of the C-U base pair was also determined by NOE-based structure calculation. From the solution structure of the RNA oligomer, r[CGACUCAGG].r[CCUGCGUCG], it was found that a single C-U mismatch preferred being stacked in the duplex rather than being flipped-out even in solution. Moreover, it adopts an irregular geometry, where the amino nitrogen (N4) of the cytidine and keto-oxygen (O4) of the uridine are within hydrogen-bonding distance, as seen in crystals. To further prove the presence of a hydrogen bond in the C-U pair, we employed a point-labeled cytidine at the exocyclic amino nitrogen of the cytidine in the C-U pair. The temperature profile of its (15)N chemical shift showed a sigmoidal transition curve, indicating the presence of a hydrogen bond in the C-U pair in the duplex.
About this Structure
Full crystallographic information is available from OCA.
Reference
Solution structure of an RNA duplex including a C-U base pair., Tanaka Y, Kojima C, Yamazaki T, Kodama TS, Yasuno K, Miyashita S, Ono A, Ono A, Kainosho M, Kyogoku Y, Biochemistry. 2000 Jun 20;39(24):7074-80. PMID:10852704 Page seeded by OCA on Fri May 2 12:19:17 2008
