5hsa

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'''Unreleased structure'''
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{{Large structure}}
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==Alcohol Oxidase AOX1 from Pichia Pastoris==
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<StructureSection load='5hsa' size='340' side='right' caption='[[5hsa]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5hsa]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HSA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HSA FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAS:ARABINO-FLAVIN-ADENINE+DINUCLEOTIDE'>FAS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alcohol_oxidase Alcohol oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.13 1.1.3.13] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hsa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hsa OCA], [http://pdbe.org/5hsa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hsa RCSB], [http://www.ebi.ac.uk/pdbsum/5hsa PDBsum]</span></td></tr>
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</table>
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{{Large structure}}
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== Function ==
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[[http://www.uniprot.org/uniprot/ALOX1_KOMPC ALOX1_KOMPC]] Major isoform of alcohol oxidase, which catalyzes the oxidation of methanol to formaldehyde and hydrogen peroxide, the first step in the methanol utilization pathway of methylotrophic yeasts.<ref>PMID:2657390</ref> <ref>PMID:9396748</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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FAD-dependent alcohol oxidases (AOX) are key enzymes of methylotrophic organisms that can utilize lower primary alcohols as sole source of carbon and energy. Here we report the crystal structure analysis of the methanol oxidase AOX1 from Pichia pastoris. The crystallographic phase problem was solved by means of Molecular Replacement in combination with initial structure rebuilding using Rosetta model completion and relaxation against an averaged electron density map. The subunit arrangement of the homo-octameric AOX1 differs from that of octameric vanillyl alcohol oxidase and other dimeric or tetrameric alcohol oxidases, due to the insertion of two large protruding loop regions and an additional C-terminal extension in AOX1. In comparison to other alcohol oxidases, the active site cavity of AOX1 is significantly reduced in size, which could explain the observed preference for methanol as substrate. All AOX1 subunits of the structure reported here harbor a modified flavin adenine dinucleotide, which contains an arabityl chain instead of a ribityl chain attached to the isoalloxazine ring.
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The entry 5hsa is ON HOLD
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Crystal Structure of Alcohol Oxidase from Pichia pastoris.,Koch C, Neumann P, Valerius O, Feussner I, Ficner R PLoS One. 2016 Feb 23;11(2):e0149846. doi: 10.1371/journal.pone.0149846., eCollection 2016. PMID:26905908<ref>PMID:26905908</ref>
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Authors: Neumann, P., Ficner, R., Feussner, I., Koch, C.
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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Description: Alcohol Oxidase AOX1 from Pichia Pastoris
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<div class="pdbe-citations 5hsa" style="background-color:#fffaf0;"></div>
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[[Category: Unreleased Structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Alcohol oxidase]]
[[Category: Feussner, I]]
[[Category: Feussner, I]]
[[Category: Ficner, R]]
[[Category: Ficner, R]]
[[Category: Koch, C]]
[[Category: Koch, C]]
[[Category: Neumann, P]]
[[Category: Neumann, P]]
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[[Category: Oxidoreductase]]

Revision as of 03:34, 10 March 2016

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Alcohol Oxidase AOX1 from Pichia Pastoris

5hsa, resolution 2.35Å

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