1ck4
From Proteopedia
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'''CRYSTAL STRUCTURE OF RAT A1B1 INTEGRIN I-DOMAIN.''' | '''CRYSTAL STRUCTURE OF RAT A1B1 INTEGRIN I-DOMAIN.''' | ||
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[[Category: Pepinsky, R B.]] | [[Category: Pepinsky, R B.]] | ||
[[Category: Venyaminov, S Y.]] | [[Category: Venyaminov, S Y.]] | ||
| - | [[Category: | + | [[Category: Adhesion]] |
| - | [[Category: | + | [[Category: Collagen]] |
| - | [[Category: | + | [[Category: I-domain]] |
| - | [[Category: | + | [[Category: Metal binding]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:49:17 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 09:49, 2 May 2008
CRYSTAL STRUCTURE OF RAT A1B1 INTEGRIN I-DOMAIN.
Overview
The alpha1beta1 integrin is a major cell surface receptor for collagen. Ligand binding is mediated, in part, through a 200 amino acid inserted 'I'-domain contained in the extracellular part of the integrin alpha chain. Integrin I-domains contain a divalent cation binding (MIDAS) site and require cations to interact with integrin ligands. We have determined the crystal structure of recombinant I-domain from the rat alpha1beta1 integrin at 2.2 A resolution in the absence of divalent cations. The alpha1 I-domain adopts the dinucleotide binding fold that is characteristic of all I-domain structures that have been solved to date and has a structure very similar to that of the closely related alpha2beta1 I-domain which also mediates collagen binding. A unique feature of the alpha1 I-domain crystal structure is that the MIDAS site is occupied by an arginine side chain from another I-domain molecule in the crystal, in place of a metal ion. This interaction supports a proposed model for ligand-induced displacement of metal ions. Circular dichroism spectra determined in the presence of Ca2+, Mg2+ and Mn2+ indicate that no changes in the structure of the I-domain occur upon metal ion binding in solution. Metal ion binding induces small changes in UV absorption spectra, indicating a change in the polarity of the MIDAS site environment.
About this Structure
1CK4 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the alpha1beta1 integrin I-domain: insights into integrin I-domain function., Nolte M, Pepinsky RB, Venyaminov SYu, Koteliansky V, Gotwals PJ, Karpusas M, FEBS Lett. 1999 Jun 11;452(3):379-85. PMID:10386626 Page seeded by OCA on Fri May 2 12:49:17 2008
