1ckm

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[[Image:1ckm.gif|left|200px]]
[[Image:1ckm.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1ckm |SIZE=350|CAPTION= <scene name='initialview01'>1ckm</scene>, resolution 2.5&Aring;
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The line below this paragraph, containing "STRUCTURE_1ckm", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=GPA:Active+Site+LYS'>GPA</scene> and <scene name='pdbsite=GPB:Active+Site+LYS'>GPB</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=GTP:GUANOSINE-5&#39;-TRIPHOSPHATE'>GTP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/mRNA_guanylyltransferase mRNA guanylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.50 2.7.7.50] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1ckm| PDB=1ckm | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ckm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ckm OCA], [http://www.ebi.ac.uk/pdbsum/1ckm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ckm RCSB]</span>
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}}
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'''STRUCTURE OF TWO DIFFERENT CONFORMATIONS OF MRNA CAPPING ENZYME IN COMPLEX WITH GTP'''
'''STRUCTURE OF TWO DIFFERENT CONFORMATIONS OF MRNA CAPPING ENZYME IN COMPLEX WITH GTP'''
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[[Category: Paramecium bursaria chlorella virus 1]]
[[Category: Paramecium bursaria chlorella virus 1]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: mRNA guanylyltransferase]]
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[[Category: MRNA guanylyltransferase]]
[[Category: Doherty, A J.]]
[[Category: Doherty, A J.]]
[[Category: Hakansson, K.]]
[[Category: Hakansson, K.]]
[[Category: Wigley, D B.]]
[[Category: Wigley, D B.]]
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[[Category: capping enzyme]]
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[[Category: Capping enzyme]]
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[[Category: mrna]]
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[[Category: Mrna]]
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[[Category: nucleotidyltransferase]]
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[[Category: Nucleotidyltransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:50:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:23:27 2008''
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Revision as of 09:50, 2 May 2008

Image:1ckm.gif

Template:STRUCTURE 1ckm

STRUCTURE OF TWO DIFFERENT CONFORMATIONS OF MRNA CAPPING ENZYME IN COMPLEX WITH GTP


Overview

We have solved the crystal structure of an mRNA capping enzyme at 2.5 A resolution. The enzyme comprises two domains with a deep, but narrow, cleft between them. The two molecules in the crystallographic asymmetric unit adopt very different conformations; both contain a bound GTP, but one protein molecule is in an open conformation while the other is in a closed conformation. Only in the closed conformation is the enzyme able to bind manganese ions and undergo catalysis within the crystals to yield the covalent guanylated enzyme intermediate. These structures provide direct evidence for a mechanism that involves a significant conformational change in the enzyme during catalysis.

About this Structure

1CKM is a Single protein structure of sequence from Paramecium bursaria chlorella virus 1. Full crystallographic information is available from OCA.

Reference

X-ray crystallography reveals a large conformational change during guanyl transfer by mRNA capping enzymes., Hakansson K, Doherty AJ, Shuman S, Wigley DB, Cell. 1997 May 16;89(4):545-53. PMID:9160746 Page seeded by OCA on Fri May 2 12:50:08 2008

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OCA

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