1iqp

From Proteopedia

(Difference between revisions)

Revision as of 08:51, 17 January 2018

Crystal Structure of the Clamp Loader Small Subunit from Pyrococcus furiosus

Structural highlights

1iqp is a 6 chain structure with sequence from Atcc 43587. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	rfcs (ATCC 43587)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RFCS_PYRFU] Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-dependent ATPase activity which is further stimulated by PCNA.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

In eukaryotic DNA replication, replication factor-C (RFC) acts as the clamp loader, which correctly installs the sliding clamp onto DNA strands at replication forks. The eukaryotic RFC is a complex consisting of one large and four small subunits. We have determined the crystal structure of the clamp loader small subunit (RFCS) from Pyrococcus furiosus. The six subunits, of which four bind ADP in their canonical nucleotide binding clefts, assemble into a dimer of semicircular trimers. The crescent-like architecture of each subunit formed by the three domains resembles that of the delta' subunit of the E. coli clamp loader. The trimeric architecture of archaeal RFCS, with its mobile N-terminal domains, involves intersubunit interactions that may be conserved in eukaryotic functional complexes.

Atomic structure of the clamp loader small subunit from Pyrococcus furiosus.,Oyama T, Ishino Y, Cann IK, Ishino S, Morikawa K Mol Cell. 2001 Aug;8(2):455-63. PMID:11545747^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cann IK, Ishino S, Yuasa M, Daiyasu H, Toh H, Ishino Y. Biochemical analysis of replication factor C from the hyperthermophilic archaeon Pyrococcus furiosus. J Bacteriol. 2001 Apr;183(8):2614-23. PMID:11274122 doi:http://dx.doi.org/10.1128/JB.183.8.2614-2623.2001
↑ Matsumiya S, Ishino S, Ishino Y, Morikawa K. Physical interaction between proliferating cell nuclear antigen and replication factor C from Pyrococcus furiosus. Genes Cells. 2002 Sep;7(9):911-22. PMID:12296822
↑ Oyama T, Ishino Y, Cann IK, Ishino S, Morikawa K. Atomic structure of the clamp loader small subunit from Pyrococcus furiosus. Mol Cell. 2001 Aug;8(2):455-63. PMID:11545747

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1iqp"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of the Clamp Loader Small Subunit from Pyrococcus furiosus==
 <StructureSection load='1iqp' size='340' side='right' caption='[[1iqp]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rfcs ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2261 ATCC 43587])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1iqp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iqp OCA], [http://pdbe.org/1iqp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1iqp RCSB], [http://www.ebi.ac.uk/pdbsum/1iqp PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1iqp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iqp OCA], [http://pdbe.org/1iqp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1iqp RCSB], [http://www.ebi.ac.uk/pdbsum/1iqp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1iqp ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 13: / Line 14: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iq/1iqp_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iq/1iqp_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

1iqp

From Proteopedia

Revision as of 08:51, 17 January 2018

Crystal Structure of the Clamp Loader Small Subunit from Pyrococcus furiosus

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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